A study on the host specificity of rhizobia to leguminous plants

本研究旨在阐明根瘤菌对豆科植物的寄主专一性。这项研究是1992年至1993年进行的。1992年，从世界各地收集了各种根瘤形成细菌。从90种植物中分离得到约150株菌株。根据16S rRNA序列对它们进行了鉴定，并进行了系统发育分析。16SrRNA序列分析表明，豆科植物的进化与根瘤菌的进化没有相关性。1993年，进行了茎节固氮根瘤菌结瘤相关基因的分离。最后，分离出了nod M军团并进行了测序。为了建立豆科植物转座子标记方法，对几种豆科植物愈伤组织的再生能力进行了研究。

This study aimed to clarify the host specificity of rhizobia to leguminous plants. This study was carried out from 1992 to 1993. In 1992 various root-nodule forming bacteria were collected from all over the world. Approximately 150 strains were collected which were isolated from 90 plant species. They were identified based on their 16S rRNA sequences, and phylogenetic analysis was carried out. With respect to the 16S rRNA sequences 17 varieties were found. No correlation was found between the evolution of leguminous plants and the evolution of rhizobia. In 1993 isolation of nodulation-associated genes of Azorhizobiuim caulinodans was carried out. Finally the nod M legion was isolated and sequenced. In order to establish the transposon tagging method for leguminous plants, regeneration ability from callus was examined for several kinds of leguminous plant species.