ADP-ribosylation of p33 (mim-1 protein) and its effect on differentiation and growth of blood cells

p33（mim-1蛋白）的ADP-核糖基化及其对血细胞分化和生长的影响

• 批准号: 04807016
• 负责人: SHIMOYAMA Makoto
• 金额: $ 1.22万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807016/
• 关键词: p33; mim-1; ADP-ribosylation; leukocyte; myeloid leukemia; post-translational modification

Arginine-specific ADP-ribosyltransferase catalyzes transfer of the ADP-ribose moiety from NAD to arginine residue(s) of target proteins. We observed the transferase and its target protein p33 in chicken bone-marrow cells and polymorphonuclear cells. Analysis of the amino acid sequence of p33 revealed that the protein was completely identical with the regions of the sequence deduced from mim-1 (myb-induced myeloid protein-1). The levels of p33 in bone-marrow celle from 6-week-old chiken were much higher than those of peripheral polymorphonuclear cells from the same chiken. The bone-marrow cells were cultured in CO_2 at 37ﾟC in growth medium containing 8% fetal calf serum and 2% heat-inactivated chiken serum. p33 and the transferase were detected in non-adherent cell fraction containing promyelocytes and polymorphonuclear cells but not in adherent cell fraction not containing those cells. These results indicate that p33 and the transferase may participate in the differentiation of bone-marrow cells to granulocytes. Auto-ADP-ribosylation of the transferase purified from chiken peripheral polymorphonuclear cells was investigated. The ADP-ribose-transferase linkage was labile in 0.5 M hydoroxylamine. The auto-ADP-ribosylated transferase showed higher activity than did the unmodified transferase in catalyzing ADP-ribosylation of the basic acceptor such as poly (L-arginine) and p33 while to ADP-ribosylate the acidic proteins such as casein, the modified transferase was less active. Thus, auto-modification of the transferase apparently alters the specificity of its own substrate.

精氨酸特异性ADP-核糖基转移酶催化ADP-核糖部分从NAD转移到靶蛋白的精氨酸残基。我们在鸡骨髓细胞和多形核细胞中观察了转移酶及其靶蛋白p33。p33的氨基酸序列分析表明，该蛋白与mim-1（myb诱导的髓样蛋白-1）推导的序列区域完全相同。6周龄鸡骨髓策勒细胞p33表达水平明显高于外周血多形核细胞。骨髓细胞在含8%胎牛血清和2%热灭活鸡血清的生长培养基中于37 ℃ CO_2中培养。在含有早幼粒细胞和多形核细胞的非贴壁细胞部分中检测到p33和转移酶，但在不含有这些细胞的贴壁细胞部分中未检测到。这些结果表明，p33和转移酶可能参与骨髓细胞向粒细胞的分化。研究了从鸡外周血多形核细胞中纯化的转移酶的自身ADP核糖基化。ADP-核糖转移酶连接在0.5 M羟胺中不稳定。自ADP-核糖基化转移酶在催化碱性受体如聚（L-精氨酸）和p33的ADP-核糖基化方面比未修饰的转移酶表现出更高的活性，而对于ADP-核糖基化酸性蛋白如酪蛋白，修饰的转移酶活性较低。因此，转移酶的自身修饰显然改变了其自身底物的特异性。

项目成果

Masaharu Terashima: "ADP-ribosylation of actins by arginine-specific ADP-ribosyltransferase purified from chicken heterophils" Eur.J.Biochem.204. 301-311 (1992)

Masaharu Terashima：“通过从鸡异嗜细胞中纯化的精氨酸特异性 ADP-核糖基转移酶对肌动蛋白进行 ADP-核糖基化”Eur.J.Biochem.204。

Kazuo Yamada: "p33,an endogenous target protein for arginine-specific ADP-ribosyltransferase in chicken polymorphonuclear leukocytes,is highly homologous to mim-1 protein(myb-induced myeloid protein-1)" FEBS Lett.311. 203-205 (1992)

Kazuo Yamada：“p33 是鸡多形核白细胞中精氨酸特异性 ADP-核糖基转移酶的内源性靶蛋白，与 mim-1 蛋白（myb 诱导的骨髓蛋白-1）高度同源” FEBS Lett.311。

Shusaku Doi: "Loss of poly (ADP-riboss) synthetase activity without change in arginine-specific ADP-ribosyltransferase activity by acid-treatment" Biochem.Biophys.Acta. 1162-1/2. 115-120 (1993)

Shusaku Doi：“通过酸处理，聚（ADP-核糖）合成酶活性丧失，但精氨酸特异性 ADP-核糖基转移酶活性没有变化”Biochem.Biophys.Acta。

Kazuo Yamada: "Automodification of arginine-specific ADP-ribosyltransferase purified from chicken peripheral heterophils and alteration of the transferase activity" Arch.Biochem.Biophys.308-1. 31-36 (1994)

Kazuo Yamada：“从鸡外周异嗜细胞中纯化的精氨酸特异性 ADP-核糖基转移酶的自动修饰和转移酶活性的改变”Arch.Biochem.Biophys.308-1。

Marek Banasik: "Specific inhibitors of poly(ADP-ribose)synthetase and mono(ADP-ribosyl)transferase" J.Biol.Chem.267. 1569-1575 (1992)

Marek Banasik：“聚（ADP-核糖）合成酶和单（ADP-核糖基）转移酶的特异性抑制剂”J.Biol.Chem.267。