ADP-Ribosylation Reaction in Rabbit Skeletal Muscle SR

兔骨骼肌 SR 中的 ADP-核糖基化反应

• 批准号: 01580192
• 负责人: SHIMOYAMA Makoto
• 金额: $ 1.22万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01580192/
• 关键词: mono (ADP-ribose); NAD; ADP-ribosyltransferase; skeletal muscle SR; posttranslational modification; SR membrane; arginine; モノ(ADP-リボ-ス); 後翻訳修飾

Arginine-specific mono (ADP-ribosyl) transference in rabbit skeletal muscle sarcoplasmic reticulum (SR) was investigated. The enzyme solubilized with 1% Triton X-100 from the SR membranes was purified and characterized. Trypsin also solubilized the transference as active form. The properties including the molecular sizes of the trypsin-solubilized enzyme resemble those of the Triton-solubilized enzyme. These results indicate that just one part of the transference may be inserted in the SR membrane.Studies on distribution of the transference in chickens showed the highest specific activity in peripheral polymorphonuclear cells (heterophils). Purification and characterization of the heterophil enzyme revealed that the properties were indistinguishable from those of the liver enzyme, but those were different between chicken liver and rabbit SR enzymes. For example, dithiothreitol, which activate the liver enzyme, inhibited the SR enzyme. The physiological role of endogenous transference remains obscure, presumably because much less is known of acceptor proteins for the enzyme. We identified and characterized them ; Ca^<2+> -ATPase in rabbit skeletal muscle SR and 33 kDa protein (p33) in chicken liver. Chicken heterophils also contained high levels of p33. The SR transference did not modify the p33 and the liver enzyme did not ADP-ribosylate the ATPase. These results suggest that species, tissue and cell-specific ADP-ribosyltransferases prefer their own target proteins for the in situ ADP-ribosylation.

研究了精氨酸特异性单（ADP-核糖基）在兔骨骼肌肌浆网（SR）中的转运。用1%Triton X-100从SR膜溶解的酶被纯化和表征。胰蛋白酶也溶解了作为活性形式的转移。胰蛋白酶溶解的酶的性质，包括分子大小类似于那些的Triton溶解的酶。这些结果表明，转移的一部分可能插入SR膜。对转移在鸡体内分布的研究表明，在外周多形核细胞（嗜异性细胞）中比活性最高。异嗜性酶的纯化和性质的研究表明，这些性质是不可区分的肝酶，但这些是不同的鸡肝和兔SR酶。例如，激活肝酶的二硫苏糖醇抑制SR酶。内源性转移的生理作用仍然模糊不清，大概是因为对这种酶的受体蛋白知之甚少。我们鉴定并鉴定了它们：兔骨骼肌SR中的Ca^<2+> -ATPase和鸡肝中的33 kDa蛋白（p33）。鸡嗜异性粒细胞也含有高水平的p33。SR转移不修饰p33，肝酶不使ATP酶发生ADP-核糖基化。这些结果表明，物种，组织和细胞特异性ADP-核糖基转移酶更喜欢自己的靶蛋白的原位ADP-核糖基化。

项目成果

下山 誠: "内在性ADPリボシン転移酵素と標的タンパク質" 生化学. 63. 123-129 (1991)

Makoto Shimoyama：“内源性 ADP 核糖转移酶和靶蛋白”生物化学 63. 123-129 (1991)。

Makoto Shimoyama: "Endogenous ADP-ribosyltransference and their target proteins (Minireview)" Seikagaku. 63. 123-129 (1991)

Makoto Shimoyama：“内源性 ADP-核糖基转移及其靶蛋白（迷你评论）”Seikagaku。

Hisae Kawamitsu: "ADP-ribosylation of human cHa-ras proteins by hen liver ADP-ribosyltransferase" Niacin Nutrition, ADP-ribosylation. Springer-Verlag. 527 (1989)

Hisae Kawamitsu：“母鸡肝脏 ADP-核糖基转移酶对人类 cHa-ras 蛋白进行 ADP-核糖基化”烟酸营养，ADP-核糖基化。

Makoto SHIMOYAMA: "ADPーribosylation and endogenous acceptor proteins in animal muscle tissues. In:Niacin Nutrition,ADPーribosylation,and Cancer (M.K.Jacobson and E.J.Jacobson,eds.)" SpringerーVerlag, 527(6) (1989)

Makoto SHIMOYAMA：“动物肌肉组织中的 ADP-核糖基化和内源性受体蛋白。见：烟酸营养、ADP-核糖基化和癌症（M.K.Jacobson 和 E.J.Jacobson，编辑）”Springer-Verlag，527(6) (1989)

Makoto Shimoyama: "DNA-regulated mono (ADP-ribosyl) ation and de-ADP-ribosylation of endogenous acceptor proteins in polymorphonuclear leukocytes" The Biology and Medicine of Signal transduction. 7th International Conference on Cyclic Nucleotides, Calcium

Makoto Shimoyama：“多形核白细胞中内源性受体蛋白的 DNA 调节单（ADP-核糖基）化和去 ADP-核糖基化”信号转导的生物学和医学。