A study of Pathogenesis in Ulcerative Colitis with Special Reference to Sulfated Polysaccharide, Intestinal Microflora and Mucosal Immunity

我们用吖啶橙染色方法研究了溃疡性结肠炎粘膜病变和细菌侵袭的相互作用。在16例溃疡性结肠炎中，粘膜均有小杆状物侵犯。在10例对照组中，5例仅见细菌附着在粘膜上，其余5例均未见细菌。提示结肠粘膜中细菌的存在似乎与溃疡性结肠炎粘膜病变的持续和加重有关。我们先前报道，在葡聚糖硫酸钠诱导的小鼠实验性溃疡性结肠炎中，大量吞噬葡聚糖硫酸钠的巨噬细胞聚集在粘膜病变和脾中。本文观察了经3%葡聚糖硫酸钠处理6个月的小鼠脾巨噬细胞的细菌吞噬能力。在该模型中，吞噬细菌(单核细胞增生性李斯特菌)数量和吞噬指数显著降低。用葡聚糖硫酸钠与小鼠脾巨噬细胞体外孵育和添加细菌的方法，检测未处理小鼠脾巨噬细胞的吞噬能力。吞噬细菌数和吞噬指数也显著降低。这些观察表明，在这个模型中，吞噬葡聚糖硫酸钠的巨噬细胞的细菌吞噬能力下降，表明粘膜对细菌的防御系统下降。

We investigated interactions berween mucosal lesions and bacterial invasion in ulcerative colitis using the acridine-orange staining method. In all 16 cases of ulcerative colitis, the mucosa was found to be invaded by small rods. In 5 of 10 controls, bacteria were seen only adhering to the mucosa and no bacteria were detected in the 5 remaining cases. It is suggested that the presence of bacteria in the colonic mucosa seems to be concerned with the persistence and aggravation of the mucosal lesion of ulcerative colitis.We previously reported that numerous macrophages which phagocytosed dextran sulfate sodium were observed to have accumulated in the mucosal lesions and in the spleen in experimental ulcerative colitis induced in mice with dextran sulfate sodium. In this paper, we investigated the bacterial phagocytic abitity of macrophages which were isolated from spleens of mice treated with 3% dextran sulfate sodium for 6 months. In this model, the number of phagocytosed bacteria (Listeria monocytogenes) and the phagocytic index were significantly decreased. The phagocytic ability of splenic macrophages obtained from non-treated mice was also evaluated by incubating with dextran sulfate sodium in vitro, and adding bacteria. The number of phagocytosed bacteria and the phagocytic index were also signigicantly decreased. These observations suggest that the decrease in bacterial phagocytosis in this model by macrophages that phagocytosed dextran sulfate sodium indicates a decline of the mucosal defense system to bacteria.