A study of Pathogenesis in Ulcerative Colitis with Special Reference to Sulfated Polysaccharide, Intestinal Microflora and Mucosal Immunity

溃疡性结肠炎发病机制的研究，特别涉及硫酸多糖、肠道菌群和粘膜免疫

• 批准号: 04807049
• 负责人: OHKUSA Toshifumi
• 金额: $ 1.41万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807049/
• 关键词: Ulcerative Colitis; Bacterial culture of the Mucosa; Intestinal Microflora; Vero toxin; Experimental Ulcerative Colitis; Dextran Sulfate Sodium; Macrophage; Immunoglobulin; コレラ菌毒素; 粘膜局所墓免疫; 腸内細菌

We investigated interactions berween mucosal lesions and bacterial invasion in ulcerative colitis using the acridine-orange staining method. In all 16 cases of ulcerative colitis, the mucosa was found to be invaded by small rods. In 5 of 10 controls, bacteria were seen only adhering to the mucosa and no bacteria were detected in the 5 remaining cases. It is suggested that the presence of bacteria in the colonic mucosa seems to be concerned with the persistence and aggravation of the mucosal lesion of ulcerative colitis.We previously reported that numerous macrophages which phagocytosed dextran sulfate sodium were observed to have accumulated in the mucosal lesions and in the spleen in experimental ulcerative colitis induced in mice with dextran sulfate sodium. In this paper, we investigated the bacterial phagocytic abitity of macrophages which were isolated from spleens of mice treated with 3% dextran sulfate sodium for 6 months. In this model, the number of phagocytosed bacteria (Listeria monocytogenes) and the phagocytic index were significantly decreased. The phagocytic ability of splenic macrophages obtained from non-treated mice was also evaluated by incubating with dextran sulfate sodium in vitro, and adding bacteria. The number of phagocytosed bacteria and the phagocytic index were also signigicantly decreased. These observations suggest that the decrease in bacterial phagocytosis in this model by macrophages that phagocytosed dextran sulfate sodium indicates a decline of the mucosal defense system to bacteria.

我们使用吖啶橙染色法研究溃疡性结肠炎中粘膜病变和细菌侵袭之间的相互作用。16例溃疡性结肠炎均可见小杆状物侵入粘膜。在10例对照中的5例中，仅观察到细菌粘附在粘膜上，在其余5例中未检测到细菌。我们曾报道，在用葡聚糖硫酸钠诱发的小鼠溃疡性结肠炎模型中，大量吞噬葡聚糖硫酸钠的巨噬细胞聚集在溃疡性结肠炎的粘膜病变和脾脏中。本文观察了3%葡聚糖硫酸钠处理6个月的小鼠脾巨噬细胞对细菌的吞噬能力。在该模型中，吞噬细菌（单核细胞增生李斯特菌）的数量和吞噬指数显著降低。还通过与葡聚糖硫酸钠体外孵育并加入细菌来评价从未处理小鼠获得的脾巨噬细胞的吞噬能力。吞噬细菌数和吞噬指数也明显降低。这些观察结果表明，在该模型中，吞噬葡聚糖硫酸钠的巨噬细胞的细菌吞噬作用降低，表明粘膜防御系统对细菌的防御能力下降。

项目成果

T.Ohkusa,I.Okayasu,S.Tokoi,A.Araki,Y.Ozaki: "Changes in Bacteral Phagocytosin of Macrophages in Experimental Vlcenative Colitis" Digestion. (in print). (1994)

T.Ohkusa，I.Okayasu，S.Tokoi，A.Araki，Y.Ozaki：“实验性结肠炎巨噬细胞细菌吞噬素的变化”消化。

T.Ohkusa,I.Okayasu,S.Tokoi,A.Araki,Y.Ozaki.: "Changes in Bacterial Phagocytosis of Macrophages in Experimental Ulcerative Colitis." Digestion. (in print). (1994)

T.Ohkusa，I.Okayasu，S.Tokoi，A.Araki，Y.Ozaki.：“实验性溃疡性结肠炎巨噬细胞吞噬作用的变化。”

Ohkusa T, Okayasu I, Tokoi S, Araki A, Ozaki Y.: "Changes in bacterial phagocytosis of macrophages in experimental ulcerative colitis" Digestion. (in print.). (1994)

Ohkusa T、Okayasu I、Tokoi S、Araki A、Ozaki Y.：“实验性溃疡性结肠炎中巨噬细胞细菌吞噬作用的变化”消化。

T.Ohkusa,I.Okayasu,S.Tokoi,Y.Ozaki: "Bacterial invasion into the colonic mucosa in ulcerative colitis" Journal of Gastroenterology and Hepatology. 8. 116-118 (1993)

T.Ohkusa，I.Okayasu，S.Tokoi，Y.Ozaki：“溃疡性结肠炎中细菌侵入结肠粘膜”胃肠病学和肝病学杂志。

Ohkusa T, Okayasu I, Tokoi S, Ozaki Y.: "Bacterial invasion into the colonic mucosa in ulcerative colitis" Journal of Gastroenterology and Hepatology. 8. 116-118 (1993)

Ohkusa T、Okayasu I、Tokoi S、Ozaki Y.：“溃疡性结肠炎中细菌侵入结肠粘膜”胃肠病学和肝病学杂志。