Corticotropin releasing factor secretion by dendritic cells in response to stimulation by commensal

背景：促肾上腺皮质激素释放因子(CRF)影响肠运动功能和内脏感觉，可能参与了IBS的发病机制。肠粘膜中的树突状细胞似乎是肠道免疫系统中的关键角色，但据我们所知，还没有关于这些细胞分泌CRF的报道。方法：将小鼠树突状细胞系JAWSII细胞(ATCC CRL-11904)接种于24孔培养板中，培养3d。向细胞中加入梭状芽胞杆菌(JCM1291)、普通芽孢杆菌(JCM5856)、大肠杆菌(JCM1649)、变种杆菌(ATCC8501)共生菌株，培养2小时。结果：对照组DC分泌CRF为40.4 pg/ml，而变种镰刀菌对CRF蛋白和mRNA表达的上调作用最大。结论：DC产生CRF是一种共生菌促进的过程，其机制可能与IBS的发生有关。

Background : Corticotropin releasing factor (CRF) influencing gut motor function and visceral perception appears to be involved in the pathogenesis of IBS. Dedritic cells in the intestinal mucosa seem to be key players in the intestinal immune system, but to our knowledge, there is no report on the secretion of CRF from these cells. In this study, we investigated whether CRF secretion occurred in dendritic cells, and if so, whether or not intestinal mucosal bacteria could stimulate and increase its secretion from the cells.Methods : JAWSII cells (ATCC CRL-11904), a mouse dendritic cell line, were seeded into 24-well culture plates and grown for three days. Commensal bacterial strains of C.clostridiiforme (JCM1291), B.vulgatus (JCM5856) E. coli (JCM1649), or F.varium (ATCC8501) were added to the cells, and incubated for two hours. After incubation, we performed ELISA for the cultured medium, and RT-PCR for the DCs, and compared these values with controls.Results : CRF secretion by control DCs was 40.4 pg/ml and F. varium caused the greatest increase in CRF protein and mRNA expression.Conclusion: Our results suggest that DCs produce CRF, a process augmented by commensal bacteria, and that this mechanism may be involved in the development of IBS.