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Autoradio graphic study of the cerebral blood flow and neuro transmitter receptor in epleptic focus using the double tracer technique

双示踪技术对癫痫病灶脑血流和神经递质受体放射自显影研究

基本信息

批准号：
04807079
负责人：
YONEMASU Yuukichi
金额：
$ 0.77万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Cerebral blood flow (CBF) and neuro transmitters (NMDA,Acetylocholine, GABA,Benzodiazepine, 5-HT) in kainic acid induced epilepsy models were studied by multiple tracer auto radio graphy. The kainic acid (KA), 2 mug/mul, was injected into the left amygdala of Wistar rats through previously implanted cannula in awake and unrestrained condition. All rats with the KA injection developed limbic seizure as reported earlier. One month after the KAinjection, femoral artery and vein of the rats were cannulated under Halothane anesthesia. After recovery from tlhe anesthesia, the rats were injected with ^<14>C-IAP.Serial arterial blood samplings were performed and the rats were decapitated 30 seconds after the ^<14>C-IAP injection. The brain was quickly removed and frozen. Serial coronal sections were made with a cryostat. The CBF was measured by ^<14>C-IAP in vivo autoradio graphy. The adjacent slices were washed with Tris buffer to remove the ^<14>C-IAP.In vitro multiple tracer autoradio graphy on these adjacent sections was performed using ^3H-MK801, ^3H-QNB,^3H-Muscimol, ^3H-Ro15-1788, ^3H-5-HT.The receptor bindings were measured with image analyzer. The CBF was decreased in the left amygdala and all regions of the hippocampus. All the receptor bindings were decreased in the left amygdala and pyrmidal cell layr of the left hippocampus. However, the GABA,Benzodiazepine, 5-HT receptors were inversely increased in the fascia dentata of the hippocampus bilaterally. The changes of neuro transmitter receptor tend to be more restricted in the brain than that of CBF.Similar results were obtained from the clinical study of the human patients with temporal lobe epilepsy in our institution. Neuro imaging of the neuro transmitter receptor using the SPECT or PET scan seems to be very promissing for the localization of the epileptic focus.

应用多示踪剂放射自显影技术研究了红藻氨酸致痫模型脑血流量和神经递质（NMDA、乙酰胆碱、GABA、苯二氮卓、5-羟色胺）的变化。将海人酸（KA），2 μ g/穆尔，通过预先植入的套管在清醒和不受约束的条件下注射到Wistar大鼠的左杏仁核中。所有注射KA的大鼠均出现边缘癫痫发作，如先前报道的。KA注射后1个月，在氟烷麻醉下对大鼠的股动脉和静脉插管。麻醉恢复后，大鼠注射^<14>C-IAP，在注射^ C-IAP后30秒进行连续动脉采血并将大鼠断头<14>。大脑被迅速取出并冷冻。用低温恒温器制作连续冠状切片。采用<14>在体放射自显影技术测定脑血流量。用Tris缓冲液洗涤相邻切片以除去^<14>C-IAP，用^3 H-MK 801、^3 H-QNB、^3 H-Muscimol、^3 H-Ro 15 -1788、^3 H-5-HT对相邻切片进行体外多重示踪放射自显影，用图像分析仪测量受体结合。左侧杏仁核和海马所有区域的CBF均降低。在左侧杏仁核和左侧海马锥体细胞层，所有受体结合均减少。而双侧海马齿状筋膜内GABA、苯二氮卓、5-HT受体则呈负性增加。神经递质受体的变化往往比CBF的变化更局限于脑内。我们对颞叶癫痫患者的临床研究也得到了类似的结果。使用SPECT或PET扫描的神经递质受体的神经成像对于癫痫病灶的定位似乎非常有希望。