Proteoglycans in gingival crevicular fluid as a maker of bone destruction

龈沟液中的蛋白多糖是骨质破坏的制造者

• 批准号: 04807137
• 负责人: IWAYAMA Yukio
• 金额: $ 1.34万
• 依托单位: Asahi University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807137/
• 关键词: gingival Crebicular fluid; proteoglycan; chondoroitin sulfate; Calculus; collagen Crosslinks; Western blotting; コンドロイチン4硫酸; 歯肉; グリコサミノグリカン; 陽性荷電膜

It has been demonstrated that chondoroitin sulfate proteoglycan(CS-PG) in assosiated with th emetabolism and destruction of deerer periodontal tissue. This study was porformed to confirm the hypothesis of CS-PG as a maker of bone destruction. 1. the extracted proteins from GCF, gingiva and serum were separated by SDS-PAGE, transfered to nitrocellulose by the method of Western blotting. Immunostaining was performed using monoclonal antivodies which recognize epitopes in C4S and C6S assosiated with PG.The major bands of both PGs in GcF corresponded in electrophoretic mobikity to that of these rum, while a few faintly stained bands in GCF Correspond to that of the gingica. 2. The sulfated PGs from human subgingival calculus were separated on Cellulise acetate membrane using two dimensional electrophoresis. The results showed that CS-PG is the sole sulfated PG in decalcified Clculus. In addition, all of CS-PG was C4S-PG, not detectable C6S isomer in sample by HPLC procedure. 3. Pyridinoline(Pyr) and Deoxypyridinoline(DPyr), spesific crosslinks to bone collagen, were detected in GCF by ELISA technuque, These crosslinks were found to increase in the acute phase and decrease in the chronic phase during experimental periodontitis. Such a findings is accord with the previous results in which the content of C4S-PG in GCF increase in acute phase of inflammation.

研究表明，硫酸软骨素蛋白多糖(CS-PG)与鹿牙周组织的代谢和破坏有关。本研究旨在证实CS-PG作为骨破坏标志物的假说。1.从牙周组织、牙周组织和血清中提取的蛋白质经SDS-PAGE分离，用Western blotting方法转移到硝酸纤维素膜上。用识别与PG结合的C4S和C6S表位的单抗进行免疫染色，GCF中的两个PG的主要条带与这些朗姆酒的主要条带相对应，而GCF中的少数弱染色条带与银杏的条带相对应。2.采用双向电泳法从人的龈下结石中分离得到硫酸盐化的PGs。结果表明，CS-PG是脱钙微球中唯一的硫酸盐基PG。此外，CS-PG均为C4S-PG，样品中未检测到C6S异构体。3.用酶联免疫吸附试验检测了牙周炎患者牙周组织中与骨胶原特异性的两种交联物--吡啶酚(PYR)和脱氧吡啶酚(DPyr)，发现这些交联物在牙周炎急性期增加，慢性期减少。这一发现与以往炎症急性期GCF中C4S-PG含量升高的结果一致。

项目成果

本田 準虎: "ヒト歯肉縁上ならびに縁下歯石のグリコサミノグリカンの分離とその特性" 日本歯周病学会誌. 34. 630-632 (1993)

Juntora Honda：“从人龈上和龈下牙结石中分离糖胺聚糖及其特征”日本牙周病学会杂志 34. 630-632 (1993)。

H.honda: "****Isolation and characteriystion of glycasaminoglycans in Human supra and subginjirml caleulus" J.Japanese Soc. of periodont*l. 34. 630-632 (1993)

H.honda：“****人上和下金黄色葡萄球菌中糖胺聚糖的分离和表征”J.Japan Soc。

柴田 麻紀: "健常歯肉組織における歯肉溝滲出液及び歯肉コンドロイチン硫酸プロテオグリカン" 日本歯周病学会誌. 36(投稿予定). (1994)

Maki Shibata：“健康牙龈组织中的龈沟液和牙龈硫酸软骨素蛋白多糖”，日本牙周病学会杂志 36（待提交）。

M.Shibata: "***Chordritin sulfaste cans in **** crebi* fluid and gin* with fenlthy p***t* tissue" J.Japanese Soc. of periodont*l. 36 (in press). (1994)

M.Shibata：“***硫酸软骨素罐装在**** crebi*液体和杜松子酒*中，带有恶臭的p***t*组织”J.Japan Soc。