Responses of individual smooth muscle cells to sympathetic stimulation visualized within the arterial wall using confocal microscopy

使用共聚焦显微镜观察动脉壁内单个平滑肌细胞对交感神经刺激的反应

• 批准号: 05454146
• 负责人: IINO Masamitsu
• 金额: $ 3.84万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454146/
• 关键词: Vascular smooth muscle; Calcium ion; Blood pressure; Sympathetic nerve; Confocal microscope; カルシウムイオン

We studied the sympathetic control of vascular smooth muscle cells and obtained results that make the conventional view to be reconsidered. Using a confocal microscopy, we succeeded in visualizing [Ca^<2+>]_i change in individual smooth muscle cells in situ within the wall of rat tail artery in response to perivascular sympathtetic nerve network activities. Unlike the previous expectation that each smooth muscle cell respond with a constant [Ca^<2+>]_i rise to constant sympathetic stimulation, individual cells are shown to respond in intermittent rises in [Ca^<2+>]_i (Ca^<2+> oscillations). As the phases of Ca^<2+> oscillations differ from cell to cell, the average of many cells appear as if a constant response. Therefore, previous measurements failed to detect the oscillations. Furthermore, each oscillation propagated at a velocity of about 20 mum/s within the cell as Ca^<2+> wave due to release of Ca^<2+> from internal stores. Our results therefore demonstrate that intracellular Ca^<2+> stores play important roles in the regulation of vascular smooth muscle contraction. We have also shown clearly that Ca^<2+> oscillations are important in the regulation of cell functions.

我们研究了血管平滑肌细胞的交感神经控制，得到的结果使传统的观点重新思考。利用共聚焦显微镜，我们成功地观察了大鼠尾动脉壁内单个平滑肌细胞在血管周围交感神经网络活动下的[Ca^<2+>]_i变化。与之前的预期不同，每个平滑肌细胞对持续的交感刺激以恒定的[Ca^<2+>]_i上升作出反应，单个细胞表现为间歇性的[Ca^<2+>]_i上升（Ca^<2+>振荡）。由于Ca^<2+>振荡的相位因细胞而异，许多细胞的平均值似乎是一个恒定的响应。因此，以前的测量未能检测到振荡。此外，由于Ca^<2+>从细胞内部释放，每个振荡以大约20 μ m/s的速度在细胞内以Ca^<2+>的形式传播。因此，我们的研究结果表明，细胞内Ca^<2+>储存在血管平滑肌收缩的调节中起重要作用。我们还清楚地表明，Ca^<2+>振荡在细胞功能的调节中是重要的。

项目成果

Iino, M., Yamazawa, T., Miyashita, Y., Endo, M.and Kasai, H.: "Critical intracellular Ca^<2+> concentration for all-or-none Ca^<2+> spiking in single smooth muscle cells." EMBOJ.12. 5287-5291 (1993)

Iino, M.、Yamazawa, T.、Miyashita, Y.、Endo, M. 和 Kasai, H.：“单个平滑肌中全部或全部没有 Ca^<2> 尖峰的细胞内 Ca^<2> 临界浓度

Tsukioka,M.et al.: "pH dependence of inositol 1,4,5-trisphosphate-induced Ca^<2+> release in permeabilized smooth muscle cells of the guinea-pig." Journal of Physiology. 475. 369-375 (1994)

Tsukioka,M.等人：“豚鼠透化平滑肌细胞中肌醇 1,4,5-三磷酸诱导的 Ca^2 释放的 pH 依赖性。”

Iino,M.et al.: "Visualization of neural control of intracellular Ca^<2+> concentration in single vascular smooth muscle cells in situ." EMBO Journal. 13. 5026-5031 (1994)

Iino,M.et al.：“单个血管平滑肌细胞原位细胞内 Ca^2 浓度神经控制的可视化”。

Tsukioka, M., Iino, M.and Endo, M.: "pH dependence of inositol 1,4,5-trisphosphate-induced Ca^<2+> release in permeabilized smooth muscle cells of the guinea-pig." J.Physiol.475. 369-375 (1994)

Tsukioka, M.、Iino, M.和 Endo, M.：“豚鼠透化平滑肌细胞中肌醇 1,4,5-三磷酸诱导的 Ca^2 释放的 pH 依赖性”。

Iino,M.et al.: "Critical intracellular Ca^<2+> concentration for all-or-none Ca^<2+> spiking in single smooth muscle cells.21GC02:EMBO Journal" 12. 5287-5291 (1993)

Iino,M.et al.：“单个平滑肌细胞中全或无 Ca^<2> 尖峰的临界细胞内 Ca^<2> 浓度。21GC02：EMBO 杂志”12. 5287-5291 (1993)