Imaging analyses of signaling mechanism in central nervous system cell-cell network

我们使用信号分子的成像方法搜索了中枢神经系统（CNS）中细胞内Ca 2+信号的先前未被识别的功能，并获得了以下关于CNS中的网络机制的结果。(1)我们发现了星形胶质细胞钙信号介导的星形胶质细胞增生和神经保护机制。(2)我们成功地可视化了谷氨酸信号的时空动态。本研究扩展到在体谷氨酸成像，并证明感觉输入诱导谷氨酸溢出。(3)发现了NO诱导的Ca ~（2+）释放机制（NICR）。NICR参与小脑皮层的长时程增强和NO介导的神经元细胞死亡。

We searched for previously unidentified functions of intracellular Ca2+ signals in the central nervous system (CNS) using imaging methods for signaling molecules, and obtained the following results as regard the network mechanism in the CNS. (1) We discovered astrocytic Ca2+ signaling-mediated mechanism for astrogliosis and neuroprotection. (2) We succeeded in the visualization of spatiotemporal dynamics of glutamate signaling. This study was extended to in vivo glutamate imaging, and proved that sensory input induces glutamate spillover. (3) We discovered NO-induced Ca2+ release mechanism (NICR). NICR is involved in the long-term potentiation in the cerebellar cortex and in NO-mediated neuronal cell death.