Analysis of molecular basis for allo-recognition

同种异体识别的分子基础分析

基本信息

批准号：
05454212
负责人：
KUROSAWA Yoshikazu
金额：
$ 4.22万
依托单位：
Institute for Comprehensive Medical Science, Fujita Health Univ.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454212/
关键词：
MHC class I genes cyprinid fish elasmoblanch human MR1 gene evolution MHC遺伝子 MHCクラスI分子免疫の系統発生 PCR 遺伝子ファミリー分子進化組織特異性アロ認識

项目摘要

(1) We have analyzed three genomic genes and three cDNA clones that encode MHC class I molecules in cyprinid fishes, namely the ginbuna crucian carp and the carp. Since these six genes have been isolated by the homology of the sequence of alpha3 domain, the amino acid sequences of the alpha3 domains showed 70-90% identities among them. Based on the extent of homology, they can be classified into four classes. Each of the class consists of several to more than ten members. Thus, this group of MHC genes is composed of 30 to 40 members. Since there are several groups, there are at least 100 genes that encode MHC class I genes in cyprinid fish.(2) We have characterized the genes encoding MHC class I-like molecules in elasmoblanchs (shark).One of the genes encodes a typical class I molecule that consists of three extra-cellular domains. The amino acid sequence of alpha1 and alpha2 domains showed 40 to 50 % identities to those of human HLA and mouse H2. This result idicated that a typical MHC class I gene exists in elasmoblanchs and that the sequences of alpha1 and alpha2 domains have been kept at a high degree during evolution from elasmobranch to mammalian.(3) We have isolated a new gene that encodes MHC class I-like molecule outside of human HLA.While HLA genes are encoded at human chromosome 6, a newly isolated gene, MR1, is encoded at chromosome 1q25. The amino acid sequence of alpha1 and alpha2 of MR1 showed 40 to 50 % identities to those of HLA and mouse H2, even chicken MHC.Since we have already isolated one more gene, MR2, there should be many other genes that encode MHC class I-like genes to be analyzed in human genome. This suggested that the number of MHC genes and their diversification was much larger than expected before.

（1）我们分析了三个基因组基因和三个cDNA克隆，它们在塞普林鱼类中编码MHC I类分子，即Ginbuna Crucian Carp和Carp。由于这六个基因已通过alpha3结构域的序列的同源性分离，因此α3结构域的氨基酸序列显示出70-90％的身份。根据同源性的程度，它们可以分为四类。每个班级都由几至十多名成员组成。因此，这组MHC基因由30至40个成员组成。由于有几个组，因此至少有100个基因编码塞普林鱼中的MHC I类基因。（2）我们表征了编码MHC I类分子（鲨鱼）（鲨鱼）的基因（鲨鱼）。一个基因的一个编码一个典型的I分类分子，该分子由三个额外的胞外域组成。 α1和α2结构域的氨基酸序列对人HLA和小鼠H2的氨基酸序列显示40％至50％。 This result idicated that a typical MHC class I gene exists in elasmoblanchs and that the sequences of alpha1 and alpha2 domains have been kept at a high degree during evolution from elasmobranch to mammalian.(3) We have isolated a new gene that encodes MHC class I-like molecule outside of human HLA.While HLA genes are encoded at human chromosome 6, a newly分离的基因MR1在1q25染色体上编码。 MR1的Alpha1和Alpha2的氨基酸序列对HLA和HLA H2的氨基酸序列显示出40％至50％的身份，甚至是Chicken MHC。由于我们已经隔离了一个基因MR2，也应该有许多其他编码MHC I类基因的基因，以在人类基因组中进行分析。这表明MHC基因及其多样化的数量比以前预期的要大得多。