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Analysis of the genes in lymphocyte differentiation.

淋巴细胞分化基因分析。

基本信息

批准号：
61480478
负责人：
KUROSAWA Yoshikazu
金额：
$ 4.42万
依托单位：
FUJITA-GAKUEN HEALTH UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

We are analyzing functions of sigma structure in human immunoglobulin heavy chain gene loci: _ located downstream of enhancer, _ and _ located between and genes, _ located upstream of s_ . In this year, we carried out the following experiments.1)We isolated immunoglobulin gene from DNA of the insectivors suncus murinus, and determined its nucleotide sequence. Comparison of nucleotide sequences between man mouse and suncus will give us information for biologically meaningful regions.2. We established a B cell line expressing both IgM and IgG on the cell surface by transformation of peripheral plood cells of a patient with hyper IgM immunodeficiency using epstein-barr viruses. The data showed that in double isotype-bearing cells, one VHDHJH exon in this transript is alternatively spliced to C_ or C_ without DNA rearrangement. The defect in this disease could be at the S-S recombination stage.3) Although the frequency of IgD-secreting cells is extremely low in mouse, about 1% of the patients with myeloma produce IgD in humam. We analyzed dna from human IgD myelomas. The data indicated that homologous recombination between _ and _ sequences mediates class switch from to in human, and that it occurs via unequal crossing over between sister chromatids or daughter chromosomes.4)We are analyzing mechanism alternative rna splicing in ^- and ^- double producers.5)We are analyzing precence of discontinuous transcription followed by trans RNA splicing.

我们正在分析人免疫球蛋白重链基因座Sigma结构的功能：位于增强子下游，位于基因之间，位于S上游。在这一年里，我们开展了以下实验：1)从昆虫Suncus Murinus的DNA中分离出免疫球蛋白基因，并测定了其核苷酸序列。比较人、鼠和森科斯的核苷酸序列将为我们提供具有生物学意义的区域的信息。我们用Epstein-Barr病毒转化一例高度IgM免疫缺陷患者的外周血倍体细胞，建立了一株在细胞表面同时表达IgM和Ig G的B细胞系。结果表明，在双同型细胞中，该转录子中的一个VHDHJH外显子在没有DNA重排的情况下与C_或C__交替剪接。这种疾病的缺陷可能发生在S-S重组阶段。3)尽管在小鼠中IGD分泌细胞的频率极低，但大约1%的骨髓瘤患者在人类中会产生IGD。我们分析了人类IGD骨髓瘤的DNA。这些数据表明，在人类中，_和_序列之间的同源重组介导了类从到的转换，并且这种转换是通过姐妹染色单体或子染色体之间的不等交换发生的。4)我们正在分析^-和^-双产生物中替代RNA剪接的机制。5)我们正在分析不连续转录之后反式RNA剪接的过程。