Molecular biological approach for a dignasis and me chanism of sepsis

脓毒症诊断和机制的分子生物学方法

• 批准号: 05454364
• 负责人: UEDA Masakazu
• 金额: $ 4.35万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454364/
• 关键词: Sepsis; Severe Infection Dis.; MRSA; Cytokine on RNA; endothelin; PCR; MRSE

We established a rapid and sensitive diagnostic method for methicillinresistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients using nested polymerase chain reaction.mecA and TSST-1 genes were not detected in the blood samples of any of the 6 normal volunteers, which were negative by blood culture.In all the 12 samples from which MRSA was isolated by blood culture, mecA and TSST-1 genes were detected.In one sample from which S.epidermidis colonies grow after blood culture , mecA was detected but TSST-1 gene was not.Of 28 samples with blood cultures negative for MRSA,2 samples were still positive for mecA and TSST-1 genes.In these 2 patients, MRSA was isolated from specimens other than blood by culture.Although it took at least 48hr to identify MRSA by blood culture method, the presence of mecA and TSST-1 genes was determined by nested PCR method within only 3-4 hr after blood sampling.

应用巢式聚合酶链反应（nested polymerase chain reaction，PCR）技术建立了一种快速、敏感的诊断术后患者耐甲氧西林金黄色葡萄球菌（methicillinresistant Staphylococcus aureus，MRSA）菌血症的方法。mecA和TSST-1基因均被检测到，1例血培养后表皮葡萄球菌菌落生长的样本中，mecA基因被检测到，而TSST-1基因未被检测到; 28例MRSA血培养阴性的样本中，2例mecA和TSST-1基因仍为阳性，在这2例患者中，MRSA从血液以外的标本中培养分离，血培养法鉴定MRSA至少需要48 h，而巢式PCR法仅在采血后3-4 h内就可确定mecA和TSST-1基因的存在。

项目成果

Kitagawa et.al.: "DNA diagnosis for infections disease." Molecular biology in surgical clinics.295 (1994)

Kitakawa 等人：“感染性疾病的 DNA 诊断。”

Shito et.al.: "Activation of IL-1 and TNF genes by surgical stress" J.J.Surgical Met.Nut.28. 331 (1994)

Shito 等人：“手术应激激活 IL-1 和 TNF 基因”J.J.Surgical Met.Nut.28。

北島 政樹: "外科臨床の分子生物学:分子生物学と臨床医学、外科の接点" 中山書店, 298 (1994)

北岛正树：“临床外科中的分子生物学：分子生物学、临床医学和外科的交叉点”中山书店，298（1994）

石引久弥: "外科臨床ハンドブック(1)周術期感染の対策" 中山書店, 275 (1993)

石引久也：《临床外科手册（1）围手术期感染的对策》中山书店，275（1993）

市東 昌也: "mRNAレベルおよび血中濃度の変化からみた手術侵襲下におけるIL-l,TNFの活性化に関する検討" 外科と代謝・栄養. 28. 331-335 (1994)

Masaya Ichihigashi：“从 mRNA 水平和血液浓度变化的角度研究手术侵袭过程中 IL-1 和 TNF 的激活”《外科、代谢和营养》28. 331-335 (1994)。