Chemosensitivity Test with Collagen Gel Embedded Assaay

胶原凝胶包埋法可作为体外化疗药物敏感性试验。结果，根据体内浓度和时间曲线下面积（AUC）确定了最佳暴露浓度和频率。该方法可用于筛选乳腺癌内分泌治疗药物，并预测其在肿瘤最佳暴露条件下的抗癌效果。准确性高于我们以前使用的抗癌效果评价方法。而且，在与化疗剂和内分泌治疗剂的联合治疗中估计抗癌效率的增加。这一结果表明，乳腺癌患者通过与内分泌治疗药物的联合治疗，减少化疗药物是可能的。术后经TAM辅助治疗的患者，经本方法检测对TAM耐药者，有复发倾向，为临床治疗各种肿瘤提供了可能。

Collagen gel embedded assay was developed as in vitro chemosensitivity test according to efficiency for chemotherapy and reduce of toxicity. As a result, optimal exposure concentration and frequency were established depending on the area under the concentration and time curve (AUC) in vivo. This is concemed as pharmacokinetics of the chemotherapeutic agents, and correlated with those measured by nude mouse assay and clinical response rates of the chemotherapeutic agents.And this method can select appropriate endocrine therapeutic agents for breast cancer and predict their anticancer efficiencies under optimal counditions of tumor exposure to TAM and MPA.By staining with neutral red, accuracy is higher than the method which we used before in evaluation of the anticancer efficiency. And also, increase of anticancer efficiency is estimated in the combination therapy with chemotherapeutic agents and endocrine therapeutic agents. This result suggests that reduce of chemotherapeutic agents will be possible by the combination therapy with endocrine therapeutic agents for the patients with breast cancer. In patients who underwent postoperative adjuvant therapy with TAM,who were tested resistant to TAM by this method tended to develop recurrence.These results show possibility to clinical therapy for the various cancers.