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Chemosensitivity Test with Collagen Gel Embedded Assaay

胶原凝胶嵌入试验的化学敏感性测试

基本信息

批准号：
05454390
负责人：
HARA Satoshi
金额：
$ 2.24万
依托单位：
Kinki University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454390/
关键词：
Chemosensitivity test Collagen gel embedded assay Endocrine therapy agent Human breat cancer cell line AUC 制癌剤感受性試験乳癌細胞株 TAM MPA 至適接触条件内分泌療法

项目摘要

Collagen gel embedded assay was developed as in vitro chemosensitivity test according to efficiency for chemotherapy and reduce of toxicity. As a result, optimal exposure concentration and frequency were established depending on the area under the concentration and time curve (AUC) in vivo. This is concemed as pharmacokinetics of the chemotherapeutic agents, and correlated with those measured by nude mouse assay and clinical response rates of the chemotherapeutic agents.And this method can select appropriate endocrine therapeutic agents for breast cancer and predict their anticancer efficiencies under optimal counditions of tumor exposure to TAM and MPA.By staining with neutral red, accuracy is higher than the method which we used before in evaluation of the anticancer efficiency. And also, increase of anticancer efficiency is estimated in the combination therapy with chemotherapeutic agents and endocrine therapeutic agents. This result suggests that reduce of chemotherapeutic agents will be possible by the combination therapy with endocrine therapeutic agents for the patients with breast cancer. In patients who underwent postoperative adjuvant therapy with TAM,who were tested resistant to TAM by this method tended to develop recurrence.These results show possibility to clinical therapy for the various cancers.

根据化疗有效率和毒性降低的要求，建立了胶原胶包埋试验作为体外药敏试验。结果，根据体内浓度-时间曲线(AUC)下的面积，确定了最佳的暴露浓度和暴露频率。该方法可用于乳腺癌内分泌治疗药物的筛选，并预测其在和MPA的最佳暴露条件下的抗癌效果。通过中性红染色，该方法比以往使用的方法具有更高的准确性。此外，化疗药物和内分泌治疗药物联合治疗可提高抗癌效果。提示乳腺癌患者联合应用内分泌治疗药物可减少化疗药物的使用。在术后使用辅助治疗的患者中，用该方法检测出对耐药的患者有复发的趋势，这一结果为临床治疗各种癌症提供了可能性。