Chemosensitivity Test with Collagen Gel Embedded Assaay

胶原蛋白凝胶包埋法是一种体外化疗敏感性试验，可提高化疗效率，降低毒副作用。根据体内浓度和时间曲线下面积（AUC）确定最佳暴露浓度和频率。这与化疗药物的药代动力学有关，并与裸鼠试验和化疗药物的临床反应率相关。该方法可在肿瘤暴露于TAM和MPA的最佳条件下，选择合适的乳腺癌内分泌治疗剂，并预测其抗癌效果。用中性红染色，比以往评价抗癌效果的方法准确度高。同时，估计化疗药物和内分泌治疗药物联合治疗可提高抗癌效率。提示乳腺癌患者采用内分泌治疗药物联合治疗可以减少化疗药物的使用。在术后接受TAM辅助治疗的患者中，通过这种方法检测TAM耐药的患者倾向于复发。这些结果为临床治疗各种癌症提供了可能性。

Collagen gel embedded assay was developed as in vitro chemosensitivity test according to efficiency for chemotherapy and reduce of toxicity. As a result, optimal exposure concentration and frequency were established depending on the area under the concentration and time curve (AUC) in vivo. This is concemed as pharmacokinetics of the chemotherapeutic agents, and correlated with those measured by nude mouse assay and clinical response rates of the chemotherapeutic agents.And this method can select appropriate endocrine therapeutic agents for breast cancer and predict their anticancer efficiencies under optimal counditions of tumor exposure to TAM and MPA.By staining with neutral red, accuracy is higher than the method which we used before in evaluation of the anticancer efficiency. And also, increase of anticancer efficiency is estimated in the combination therapy with chemotherapeutic agents and endocrine therapeutic agents. This result suggests that reduce of chemotherapeutic agents will be possible by the combination therapy with endocrine therapeutic agents for the patients with breast cancer. In patients who underwent postoperative adjuvant therapy with TAM,who were tested resistant to TAM by this method tended to develop recurrence.These results show possibility to clinical therapy for the various cancers.