The action mechamisms of bioactive substances in follicular fluid related to human ovarian folliculogenesis and dysfunction

卵泡液中生物活性物质与人卵巢卵泡发生及功能障碍的作用机制

• 批准号: 05454438
• 负责人: IBUKI Yoshito
• 金额: $ 4.22万
• 依托单位: Gunma University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454438/
• 关键词: Inhibin; Activin; Follistatin; FSH receptor; LH; CG receptor; Gonadotropin; アクチビンレセプター; hCGレセプター; CAMP; mRNA

The function of ovary and folliculogenesis is regulated by both hormones and local autocrine/paracrine factors. Many kinds of bioactive substances exist in follicular fluid. Inhibin, activin and follistatin from porcine and/or bovine follicular fluid were isolated in our laboratory. We are successful in cloning of human FSH receptor, human LH/CG receptor and rat activin receptor type II,also. In order to elucidate the regulatory mechanisms of folliculogenesis, it is important to study the endocrine, paracrine and autocrine factors produced in ovary. We tried to purify bioactive substances from follicular fluid and studied functions of bioactive substances and gonadotropin receptors. Inhibin was purified to homogeniety from human follicular fluid with an immunoaffinity chromatography though it's concentration was much lower in human follicular fluid. The study of activin on gonodotropin receptors demonstrated that activin-A enhanced the FSH receptor mRNA and the FSH induced LH/CG receptor mRNA.We also demonstrated that actvin was the strongest activator of follistatin production and that both cAMP and PMS facilitated follistatin production.

卵巢和卵泡发生的功能受到激素和局部自分泌/旁分泌因子的调节。卵泡液中存在多种生物活性物质。我们实验室从猪和/或牛卵泡液中分离出抑制素、激活素和卵泡抑素。我们还成功克隆了人FSH受体、人LH/CG受体和大鼠II型激活素受体。为了阐明卵泡发生的调控机制，研究卵巢产生的内分泌、旁分泌和自分泌因子具有重要意义。我们尝试从卵泡液中纯化生物活性物质，并研究生物活性物质和促性腺激素受体的功能。尽管抑制素在人卵泡液中的浓度要低得多，但通过免疫亲和层析将人卵泡液中的抑制素纯化至同质。激活素对促性腺激素受体的研究表明，激活素-A 增强 FSH 受体 mRNA 和 FSH 诱导的 LH/CG 受体 mRNA。我们还证明，激活素是卵泡抑素产生的最强激活剂，cAMP 和 PMS 均促进卵泡抑素产生。

项目成果

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Minegishi T.: "structure and regulation of LH/hCG receptor." Endocrine Journal. 40. 275-287 (1993)

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Nakamura K., Nakamura M., Igarashi S., Miyamoto K., Eto Y., Ibuki Y., and Minegighi T.: "Effect of activin on LH/hCG receptor messenger ribonucleic acid in granulosa cells" Endocrinology. 34. 2329-2335 (1994)

Nakamura K.、Nakamura M.、Igarashi S.、Miyamoto K.、Eto Y.、Ibuki Y. 和 Minegighi T.：“激活素对颗粒细胞中 LH/hCG 受体信使核糖核酸的影响”内分泌学。