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The role of prostatic interstitium for expression of the obstructive symptom and prostatic growth of benign prostatic hyperplasia

前列腺间质在良性前列腺增生梗阻症状表现及前列腺生长中的作用

基本信息

批准号：
05454432
负责人：
MORIYAMA Nobuo
金额：
$ 3.39万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

The adrenergic stimulation induces the contraction of the hyperplastic tissues via a_l adrenoceptor and pharmacological studies suggested the existence of its subtypes. Recently three subtypes (a_<la>, a_<lb>, a_<ld>) have been detected and cloned using molecular biologic technique. Using the probes for these subtypes, we demonstrated their expression in cases with benign prostatic hypertplasia (BPH) and non-BPH tissues with in situ hybridization method. To quantify the ratio among these subtypes, RNase protection assay was also attempted in these cases. The expression of the a_<la> and a_<ld> adrenoceptors was diffusely reconginzed in the smooth muscles in the interstitium, while not in the glandular epithelial cells. On the contrary, the a_<lb> adrenoceptor was very weakly recognized. In all tissue samples tested, the predominant subtype mRNA was alpha1a. The total abundance of alpha1 mRNA in BPH samples was over six times that in non-BPH samples, the increased expression of alpha1a mRNA accounting for most of this increase (eight times that in non-BPH). The abundance of alpha1b did not vary between BPH and non-BPH samples, and the abundance of a ld in BPH samples was about three times in non-BPH samples. The ratio of the abundance of the subtype mRNAs, alpha1a : alpha1b : alpha1d was 63 : 8 : 29 in non-BPH samples and 85 : 1 : 14 in BPH samples.

肾上腺素刺激通过a_1肾上腺素受体诱导增生组织收缩，药理学研究表明其亚型的存在。最近使用分子生物学技术检测并克隆了三种亚型（a_<la>、a_<lb>、a_<ld>）。使用这些亚型的探针，我们通过原位杂交方法证明了它们在良性前列腺增生（BPH）和非 BPH 组织中的表达。为了量化这些亚型之间的比例，在这些情况下还尝试了 RNase 保护测定。 a_<la>和a_<ld>肾上腺素受体的表达在间质平滑肌中被广泛识别，而在腺上皮细胞中则不然。相反，a_<lb>肾上腺素受体的识别非常弱。在所有测试的组织样本中，主要的 mRNA 亚型是 alpha1a。 BPH 样本中 alpha1 mRNA 的总丰度是非 BPH 样本的六倍多，其中 alpha1a mRNA 表达量的增加占了大部分增加（非 BPH 中的八倍）。 BPH 和非 BPH 样品中 alpha1b 的丰度没有差异，BPH 样品中 ld 的丰度约为非 BPH 样品的三倍。非 BPH 样本中 alpha1a : alpha1b : alpha1d 亚型 mRNA 丰度比为 63 : 8 : 29 ，在 BPH 样本中为 85 : 1 : 14 。