Molecular endocrinological study on the regulatory mechanism of the proliferation and differentiation of female reproductive cells

在本研究中，利用northern blot杂交技术和c-myc探针分析了不同卵泡期收获的猪颗粒细胞中c-myc mRNA的表达，并比较了卵泡生长期间的差异。Northern blot分析显示，从猪小毛囊（1-2 mm）中获得的猪颗粒细胞RNA中含有丰富的2.4kb的c-myc转录物，其大小与用人类早期胎盘RNA作为对照获得的c-myc转录物相同。与小滤泡颗粒细胞RNA相比，中（3-5毫米）滤泡颗粒细胞或大（6-11毫米）滤泡颗粒细胞的总RNA制备均未显示c-myc探针可检测到的转录本。这些结果表明，c-myc mRNA在猪颗粒细胞中的表达仅在卵泡生长的早期受到限制。卵泡早期颗粒细胞中c-myc mRNA的分期特异性表达可能在卵泡早期未成熟颗粒细胞的自主生长中发挥生理作用，似乎不依赖于垂体促性腺激素刺激。

In the present study, the expression of c-myc mRNA in porcine granulosa cells harvested from varying follicle stages was analyzed using northern blot hybridization with a c-myc probe, and compared during follicular growth. Northern blot analysis revealed that porcine granulosa cell RNA obtained from small (1-2 mm) follicles contained an abundance of c-myc transcript of 2.4kb, the size of which was identical to the c-myc transcript obtained with human early placental RNA used as the control. In contrast to small-follicle granulosa cell RNA,total RNA preparations from either medium (3-5 mm) -follicle granulosa cells or large (6-11 mm) -follicle granulosa cells exhibited no detectable transcripts with the c-myc probe. These results suggest that expression of c-myc mRNA in porcine granulosa cells is limited only at the early stage of follicular growth. The stage-specific expression of c-myc mRNA in granulosa cells early in follicular growth may play a physiological role in the autonomous growth of immature granulosa cells in early folliculogenesis, seemingly independent of pituitary gonadotropic stimulation.