Molecular endocrinological study on the regulatory mechanism of the proliferation and differentiation of female reproductive cells

本研究以c-myc为探针，采用Northern杂交技术，分析了不同卵泡发育阶段猪颗粒细胞中c-myc基因的表达，并比较了不同发育阶段猪卵泡颗粒细胞中c-myc基因的表达。Northern印迹分析表明，从小卵泡(1-2 mm)中获得的猪颗粒细胞RNA含有2.4kb的c-myc转录本，其大小与人早期胎盘RNA的转录本相同。与小卵泡颗粒细胞RNA相反，无论是中等(3-5 mm)卵泡颗粒细胞还是大(6-11 mm)卵泡颗粒细胞，c-myc探针均未检测到可检测到的转录本。这些结果表明，c-myc基因在猪颗粒细胞中的表达仅限于卵泡发育的早期阶段。C-myc mRNA在卵泡发育早期颗粒细胞中的阶段特异性表达可能在卵泡发生早期未成熟颗粒细胞的自主生长中发挥生理作用，似乎不依赖于垂体促性腺激素的刺激。

In the present study, the expression of c-myc mRNA in porcine granulosa cells harvested from varying follicle stages was analyzed using northern blot hybridization with a c-myc probe, and compared during follicular growth. Northern blot analysis revealed that porcine granulosa cell RNA obtained from small (1-2 mm) follicles contained an abundance of c-myc transcript of 2.4kb, the size of which was identical to the c-myc transcript obtained with human early placental RNA used as the control. In contrast to small-follicle granulosa cell RNA,total RNA preparations from either medium (3-5 mm) -follicle granulosa cells or large (6-11 mm) -follicle granulosa cells exhibited no detectable transcripts with the c-myc probe. These results suggest that expression of c-myc mRNA in porcine granulosa cells is limited only at the early stage of follicular growth. The stage-specific expression of c-myc mRNA in granulosa cells early in follicular growth may play a physiological role in the autonomous growth of immature granulosa cells in early folliculogenesis, seemingly independent of pituitary gonadotropic stimulation.