The role of growth factors and oncogenes in the functional regulation of the ovary and placenta

已证实甲状腺激素与FSH有协同作用，对颗粒细胞功能有刺激作用。为探讨甲状腺激素对颗粒细胞作用的本质，对卵泡成熟过程中颗粒细胞胞核中T_3受体的存在进行了定性和定量的比较。用v-erb-A探针与颗粒细胞RNA进行*斑点杂交，分析erb-A的表达。小卵泡颗粒细胞的T_3受体数量高于大卵泡颗粒细胞，而小卵泡颗粒细胞比大卵泡颗粒细胞具有更强的I-T_3能力。这些结果表明，在卵泡成熟早期，甲状腺激素通过其核受体选择性地作用于未成熟的颗粒细胞，以放大颗粒细胞分化的促进剂的作用。

Thyroid hormone has been demonstrated to synergize with FSH to excite stimulatory effects on granulosa cell function. In order to characterize the nature of thyroid hormone action on granulosa cell, the presence of T3 receptors in the nuclei of granulosa cells was examined and qualitativeley and quantitativeley compared during follicular maturation. Furthermore expression of erb-A was analyzed using ***** blot hybridization of granulosa cell RNA with a v-erb-A probe. The number of T3 receptors was higher small follicle granulosa cells relative to large follicle granulosa cells, while small follicle granulosa cells had a greater ability to ^<125>I-T3 compared to those with large follicle granulosa cells. These results suggest that thyroid hormone acts via its nuclear receptors selectively in immature granule cells early in follicular maturation to amplify die actions die facilitators of granulosa cell differentiation.