The role of growth factors and oncogenes in the functional regulation of the ovary and placenta

生长因子和癌基因在卵巢和胎盘功能调节中的作用

• 批准号: 02670741
• 负责人: MARUO Takeshi
• 金额: $ 1.73万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670741/
• 关键词: Ovary; Granulosa cell; Thyroid Hormone; erb-A; maturation; Follicular Growth; Differentiation; Thyroid Hormone Receptor; ovary; plaeereta; granu losa cell; Prophoblast; epidermcl grorseh tactor; IGF-I; dual action; placenta; insulinーlike g

Thyroid hormone has been demonstrated to synergize with FSH to excite stimulatory effects on granulosa cell function. In order to characterize the nature of thyroid hormone action on granulosa cell, the presence of T3 receptors in the nuclei of granulosa cells was examined and qualitativeley and quantitativeley compared during follicular maturation. Furthermore expression of erb-A was analyzed using ***** blot hybridization of granulosa cell RNA with a v-erb-A probe. The number of T3 receptors was higher small follicle granulosa cells relative to large follicle granulosa cells, while small follicle granulosa cells had a greater ability to ^<125>I-T3 compared to those with large follicle granulosa cells. These results suggest that thyroid hormone acts via its nuclear receptors selectively in immature granule cells early in follicular maturation to amplify die actions die facilitators of granulosa cell differentiation.

甲状腺激素已被证明可以与FSH协同作用，以激发对颗粒细胞功能的刺激作用。为了表征甲状腺激素作用在颗粒细胞上的性质，检查了颗粒细胞细胞核中T3受体的存在，并在卵泡成熟过程中进行了质量和定量。此外，使用V-ERB-A探针将颗粒细胞RNA的*****印迹杂交分析ERB-A的表达。相对于大卵泡颗粒细胞，T3受体的数量是较高的小卵泡颗粒细胞，而与大卵泡颗粒细胞相比，小卵泡颗粒细胞具有更大的 ^<125> I-T3的能力。这些结果表明，甲状腺激素在卵泡成熟的早期未成熟细胞中选择性地通过其核受体作用，以扩增颗粒细胞分化的模具作用。

项目成果

Strauss JF(ed.),Maruo T,Matsuo H,LadinesーLlave CA,Katayama K,Mochizuki M: "Uterine and Emoryonic Factors in Early Pregnancy" Plenum Press,New York, 235-250 (1991)

Strauss JF（编）、Maruo T、Matsuo H、Ladines-Llave CA、Katayama K、Mochizuki M：“妊娠早期的子宫和情绪因素” Plenum Press，纽约，235-250（1991）

Maruo T, Hiramatsu S, Otani T, Mochizuki M: "Increase on die expression of thyroid hormone receptors on granulosa cells early in follicular maturation." Acta Endocrinol. 127. 152-160 (1992)

Maruo T、Hiramatsu S、Otani T、Mochizuki M：“卵泡成熟早期颗粒细胞上甲状腺激素受体的表达增加。”

Takeshi Maruo,Hiroya Matsuo,C.A.LadinesーLlave,Kazuaki Katayama & Matsuto Mochizuki,(Jerome F.Strauss,III(Ed.)): "Uterine and Embryonic Factors in Early Pregnancy(Growth factors in Placental Function)" Plenum Publishing Corporation,New York, (1991)

Takeshi Maruo、Hiroya Matsuo、C.A. Ladines-Llave、Kazuaki Katayama 和 Matsuto Mochizuki，（Jerome F. Strauss，III（编辑））：“妊娠早期的子宫和胚胎因素（胎盘功能中的生长因素）”Plenum Publishing Corporation，纽约，（1991）

LadixesーLlave CA,Maruo T,Manalo AS,Mochizuhi M.: "Cytologic localization of apidermal gcoreth tactor and its veceptor in developvng human placen to varies in the cowce of preqnamey" Amenican fournal of Obstetrics and Gynecology. 165. 1377-1382 (1991)

Ladixes－Llave CA、Maruo T、Manalo AS、Mochizuhi M.：“表皮 gcoreth 触动物及其载体在人类胎盘发育过程中的细胞学定位，在妊娠期发生变化”美国妇产科第四版 165。 1377-1382（ 1991)

Maruo T, Matsuo H, Mochizuki M: "Thyroid hormone as a biological amplify of differentiated trophoblast functions in early gestation." Acta Endocrinol. 125. 58-66 (1991)

Maruo T、Matsuo H、Mochizuki M：“甲状腺激素是妊娠早期分化滋养层功能的生物增强剂。”