Immunoresponsive Analysis of Protection against Periodontopathogen

致病菌包括伴放线放线杆菌、牙龈卟啉单胞菌和静止弯曲杆菌。本研究的目的是阐明放线菌共生放线菌菌毛附着因子的重要性以及抗菌毛抗体作为附着抑制剂的意义。从伴放线菌310-a菌株制备菌毛抗原。根据菌毛蛋白的氨基酸序列合成寡肽。将肽抗原与支链赖氨酸聚合物树脂珠缀合。将肽抗原悬浮于用不完全弗氏佐剂乳化的PBS中，并用于免疫家兔。兔抗血清可与伴放线菌310-a菌毛蛋白的约54 kDa蛋白及其它菌毛菌株的菌毛蛋白反应。该抗血清能抑制伴放线杆菌菌株与唾液包被的透明质酸的粘附， ...更多信息 用二乙基胺解离酶联免疫吸附试验检测牙周炎患者血清IgG抗体对伴放线菌和牙龈卟啉单胞菌菌毛抗原的相对亲和力。高滴度血清患者没有窝藏有显着较高的亲和力菌毛抗原比高滴度血清患者的牙周袋感染。制备了抗C.restus ATCC 33238全细胞的兔多克隆抗血清和抗C.restus ATCC 33238表面抗原的鼠单克隆抗体。抗直链梭菌ATCC 33238的150 KDa S-layr蛋白单克隆抗体与9株直链梭菌中的4株发生反应。识别150 KDa S-layr的单克隆抗体在体外呈剂量依赖性地抑制直链隐球菌与人牙龈成纤维细胞G1的粘附。豚鼠腹腔白细胞对直链梭菌ATCC 33238的吞噬作用可被兔抗血清和抗S-layr单克隆抗体所增强，但不被脂多糖单克隆抗体所增强，在无抗体条件下，直链梭菌细胞对白细胞的电镜观察显示有损伤，表明该微生物具有白细胞毒活性。少

Specific gram-negative bacteria including Actinobacillus actinomycetemcomitans, Prophyromonas gingivalis and Campylobacter restus are periodontopathogen. The purpose of this study is to critify the importance of the fimbriae attachment factor of A.actinomycetemcomitants and the significance of anti-fimbrial antibody functions as an attachment inhibitor. Fimbrial antigen was prepared from the A.actinomycetemcomitans 310-a strain. Oligopeptides were synthesized according to the amino acid sequence of the fimbrial protein. The peptide antigen was conjugated with branched lysine polymer resin beads. The peptide antigen was suspended in PBS emulsified with incomplete Freund's adjuvant and used to immunize rabbits. A rabbit antiserum reacted with an approximately 54kDa protein of the fimbriae protein from A.actinomycetemcomitans 310-a and with those of other fimbriated strains. This antiserum atrongly inhibited the attachment of fimbriated A.actinomycetemcomitans strains to saliva-coated hyd … More roxyapatite beads, buccal epithelial cells, and a fibroblast cell line, Gin-1.The relative avidity of serum immunoglobulin G antibody for the fimbria antigens from A.actinomycetemcomitans and P.gingivalis was assessed by diethylamine dissociation enzyme-linked immunosorbent assay in patients with adult periodontitis. High-titer sera from patients not harboring had significantly higher avidities for the fimbria antigen than did high-titer sera from patients with the infections in their periodontal pockets. The elicited antibodies against the fimbria antigen may afford protection against A.actinomycetemcomitans and P.gingivalis infections.Rabbit polyclonal antiserum to whole cells of C.restus ATCC33238 and monoclonal mouse antibodies against surface antigens of the microorganisms were prepared. Monoclonal antibody against 150KDa S-layr protein from C.rectus ATCC33238 reacted with 4 out of 9 C.rectus strains. The monoclonal antibody recognized 150KDa S-layr inhibited dose dependently the C.rectus attachment to human gingival fibroblast, G1 cells, in vitro. Phagocytic killing of C.rectus ATCC33238 by peritoneal leukocytes prepared from guinea pig was significantly enhanced by the rabbit antiserum and moderately enhanced by monoclonal antibody against S-layr, but was not enhanced monoclonal antibody to lipopolysaccharide.Electron micrographs of leukocytes exposed to C.rectus cells on the absence of antibody showed injury, indicating that the microorganisms possesses a leukocyte toxic activity. Less