Analysis for pathogenicity of periodontal disease-associated anaerobesin silent aspirated pneumoniae

据报道，牙周病相关细菌不仅是引起口腔感染性疾病的病原体来源，而且与全身性疾病密切相关。我们研究了牙龈卟啉单胞菌与T。在支气管肺泡灌洗液（BALF）中发现牙龈卟啉单胞菌和齿垢孢子菌混合感染后。本实验采用小鼠实验性支气管肺泡灌洗液（BALF），观察了小鼠肺泡灌洗液（BALF）中P细胞、牙龈细胞的活细胞数及炎症反应。炎性细胞因子包括白细胞介素1/β（IL-1 /β）、IL-6、肿瘤坏死因子α。根据制造商推荐的方法，通过对这些细胞因子分子特异性的ELISA系统测定BALF中的TNF 0（TNF 0）和小鼠KC（人白细胞介素8样细胞因子）。剂量为2.8 × 10^8的牙龈炎和齿垢锥虫，BALF中牙龈卟啉单胞菌的活细胞数在24、48和72 h稳定下降。P.接种T.单次感染后，龋齿发生率显著高于单次感染后（P<0.05）。感染小鼠BALF中TNF 0水平在接种后2 4 h内呈进行性升高，随后下降。混合感染组小鼠BALF中IL-1B水平在接种后24 h显著高于单纯感染组和单纯感染组。齿垢感染（P<0.05）。IL-1 B.混合感染小鼠BALF中IL-6水平随感染时间延长而降低，而IL-6水平随感染时间延长而升高。混合感染组小鼠BALF中KC水平在48和72 h均显著高于单纯感染组。齿垢引起小鼠BALF中显著的炎症反应，并导致肺水肿。

Periodontal disease-associated bacteria have been reported to be the source of pathogens that not only cause oral infectious diseases but also are closely involved in systemic diseases. We examined the inflammatory responses against mixed infections of Porphyromonas gingivalis with T. denticola in bronchoalveolar lavage fluids (BALF).After mixed infections with P. gingivalis and T. denticola into lung in mouse,we studied the viable cell numbers ofP, gingivalis cells and inflammatory responses in the BALF. Inflammatory cytokines, including interleukin 1/β (IL-1 /β), IL-6,tumor necrosis factor a. (TNF0), and mouse KC (human interleukin 8 like cytokine) were determined in BALF by ELISA systems specific for these cytokine molecules according to the manufacturer's recommended procedures.When mice were inoculated with a mixture ofP. gingivalis at a dose of2.8 x10^8 and T, denticola., viable cell numbers P. gingivalis in the BALF steadily declined at 24, 48, and 72 h. The viable cell numbers ofP. gingivalis48 h and 72 h afterinoculation of a mixed suspension with T. denticola were significantly higher thanthose after monoinfection (P<0.05). The TNF 0 level in the BALF from infected mice progressively increased for the first 24 h after intratracheal inoculatiob and then decreased. The EL-1 Blevel in the BALF in mice with mixed infections at 24h after inoculation was significantly increased as compared with that after P. gingivalis monoinfection or T. denticola infection (P<0.05). The IL-1 B. level in theBALF in the mice with mixed infections decreased with time, butthe IL-6 level in the BALF in the same mice increased with time. The KC level in the BALF in the mice with mixed infection was significantly higher than that in those with P.gingivalis monoinfection at 48 and 72 h.The present study demonstrates that a mixed inoculation of P. gingivalis withT. denticola causes a marked inflammatory response in mouse BALF and leids tolung abscesses.