The Biological Activity of Membrane Substances from Suspected Periodontal Pathogens on the Human Fibroblasts.

由于脂多糖、外膜和囊泡是所有革兰氏阴性菌的共同因子，这些革兰氏阴性菌主要存在于牙周病部位的牙龈沟中，而且它们具有明确的生物学效应，因此引起了人们的关注。本研究的目的是在标准化条件下比较脂多糖、外膜和囊泡对培养的人成纤维细胞的生物活性。刺激物来自牙周病患者的牙龈假单胞菌、中间假单胞菌、放线菌伴生菌。成纤维细胞取自同一人的健康牙龈和健康牙周韧带。对两种细胞的增殖和碱性磷酸酶活性进行了比较，结果表明：随着刺激浓度的不同，两种细胞的增殖均呈规律性变化。尤其是在三种细菌中，中间层细菌刺激后的规律性最为明显，而在囊泡刺激后，三种刺激物之间的规律性最为明显。两种细胞碱性磷酸酶活力的刺激浓度呈现相似的规律性。培养的牙周膜成纤维细胞的碱性磷酸酶活力高于牙周成纤维细胞。刺激后，对成纤维细胞进行电子显微镜观察。在本研究中，我们可以清楚地比较标准化条件下细胞的生物活性。未来，与生物活动的相关性将得到更明确的定义。

Since lipopolysaccharide (LPS), outer membrane and vesicle appear to be a common factors to all gram negative bacteria that are predominant in the gingival sulcus of the sites of periodontal disease and they have well documented biological effects, they have receipt prominent attention. The purpose of this study was to compare the biological activity under the standardized condition.We studied the effects of LPS,outer membrane and vesicle on cultured human fibroblasts. The stimulators were extracted from P.gingivalis, P.intermedia, A.actinomycetemcomitans derived from periodontal patients. Fibroblasts were obtained from healthy gingiva and healthy periodontal ligament of the same individual. The proliferation and the alkaline phosphatase activity, an osteoblastic characteristic, were compared between these two types of cell.The results were as follows :The proliferation was varied regularly on both cells according to the stimulated concentration. Particularly after the stimulation of P.intermedia regularity is most clear among the three bacteria and after the stimulation of vesicle is most clear among the three stimulators. It was showed similar regularity according to the stimulated concentration on both cells of alkaline phosphatase activity. Cultured fibroblasts derived from periodontal ligament have higher alkaline phosphatase activity than that of gingival fibroblasts. After stimulation, the fibroblasts were examined by electron microscopy. The cells showed a irregularity of the surface of outer membrane at many locations.In this study, we could clearly compare the biological activity under the standardized condition. In the future, the correlation with biological activities will be defined more clearly.