An environmental medical study on the induction of metallothionein under stressful conditions

这项研究的目的是首先确定金属硫蛋白的合成是否由各种环境应激源诱导，如噪音、振动、固定化或饥饿。这项研究的第二个目的是通过检测金属硫蛋白的性质、蛋白质亚型的组成、蛋白质在体内的分布以及组织中的变化来阐明这些蛋白质在应激条件下的作用。将生物层析系统与离子交换层析相结合，建立了异金属硫蛋白的鉴定和定量方法。我们证明了噪声、振动、束缚和饥饿可诱导大鼠肝脏中金属硫蛋白的合成和蛋白质含量的增加。

The goal of this study was firstly to determine whether the synthesis of metallothioneins was induced by various environmental stressors such as noise, vibration, immobilization, or starvation. The goal of the study was secondly to elucidate the role of the proteins on stressful conditions by examining natures of metallothioneins, composition of the isoforms of the proteins, distribution of the proteins in the body, and variations in tissues. The method for the identification and quantification of the isometallothioneins was established by the combination of the bio-chromatography system and ion-exchange chromatography. We demonstrated that the synthesis of metallothioneins was induced and the content of the protein increased in the livers of rats exposed to noise, vibration, immobilization, and starvation.