An environmental medical study on the induction of metallothionein under stressful conditions

应激条件下金属硫蛋白诱导的环境医学研究

• 批准号: 05454605
• 负责人: KOJIMA Yutaka
• 金额: $ 3.14万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454605/
• 关键词: metallothionein; metal-binding protein; stress; high-performance-liquid-chromatography; isoforms; noise; immobilization; vibration

The goal of this study was firstly to determine whether the synthesis of metallothioneins was induced by various environmental stressors such as noise, vibration, immobilization, or starvation. The goal of the study was secondly to elucidate the role of the proteins on stressful conditions by examining natures of metallothioneins, composition of the isoforms of the proteins, distribution of the proteins in the body, and variations in tissues. The method for the identification and quantification of the isometallothioneins was established by the combination of the bio-chromatography system and ion-exchange chromatography. We demonstrated that the synthesis of metallothioneins was induced and the content of the protein increased in the livers of rats exposed to noise, vibration, immobilization, and starvation.

本研究的目的是首先确定金属硫蛋白的合成是否受到各种环境应激因素（如噪音、振动、固定或饥饿）的诱导。该研究的第二目标是通过检查金属硫蛋白的性质、蛋白质同种异构体的组成、蛋白质在体内的分布和组织中的变化来阐明蛋白质在应激条件下的作用。采用生物色谱和离子交换色谱相结合的方法，建立了异金属硫肽的鉴定和定量方法。我们证明，暴露于噪音、振动、固定和饥饿的大鼠肝脏中，金属硫蛋白的合成被诱导，蛋白质含量增加。

项目成果

Yutaka Kojima 他1名: "Hygienic studies on the Federal Land Devolopment Authority(FELDA)schemes in Malaysia" J.Nor.Occ.Health. 40. (1994)

Yutaka Kojima 和其他 1 人：“马来西亚联邦土地发展局 (FELDA) 计划的卫生研究”J.Nor.Occ.Health 40。(1994)

及川伸二 他: "アルキル化剤によるDNA損傷に対するメタロチオネインの抑制機構" 日本衛生学雑誌. 49. 166 (1994)

Shinji Oikawa 等人：“金属硫蛋白对烷化剂引起的 DNA 损伤的抑制机制”，日本卫生杂志 49. 166 (1994)。

江本匡 他: "大腸菌内で発現させたセリン→ロイシン置換人工メタロチオネインの性質" 日本衛生学雑誌. 49. 435 (1994)

Tadashi Emoto 等：“大肠杆菌中表达的丝氨酸->亮氨酸取代的人工金属硫蛋白的特性”日本卫生杂志 49. 435 (1994)。

Shigeru SAITO: "Limitations of zinc and metallothionein accumulation in rat livers following zinc administration"Research Communications in Molecular Pathology and Pharmacology. VOL.88(1). 99-106 (1995)

Shigeru SAITO：“锌给药后大鼠肝脏中锌和金属硫蛋白积累的限制”分子病理学和药理学研究通讯。

Shigeru SAITO, Satoshi YOSHIZU and Yutaka KOJIMA: "Relative zinc-binding capacity of metal lothionein : Studies in renal cytosols from zinc-injected rats"Research Communications in Molecular Pathology and Pharmacology. VOL.90(3). 363-371 (1995)

Shigeru SAITO、Satoshi YOSHIZU 和 Yutaka KOJIMA：“金属硫蛋白的相对锌结合能力：注射锌大鼠肾细胞质的研究”分子病理学和药理学研究通讯。