Structural Biology of Polysialic Acid Recognition

聚唾液酸识别的结构生物学

• 批准号: 432187713
• 负责人: Professor Dr. Thilo Stehle
• 金额: --
• 依托单位: Interfakultäres Institut für Biochemie (IFIB)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/432187713?language=en
• 关键词: Structural; Biology; Polysialic; Acid; Recognition

The aim of the application is to structurally investigate the binding of polysialic acids to various cellular proteins, and to characterize these interactions using affinity measurements. The application focuses on the one hand on Siglecs (sialic acid-binding receptors and signaling molecules) and on the other hand on CXCL12-type chemokines. Both protein types bind polysialic acids, and in both cases the binding is highly relevant for the function of the respective protein. In preliminary work, we already have determined a high-resolution crystal structure of a central domain of the Siglec-11 protein, and various longer protein fragments have been expressed and purified. In terms of methods, protein biochemistry and structure elucidation (NMR spectroscopy, X-ray crystallography) as well as various affinity measurement methods will be used. The project features tight collaborations with several other projects, most importantly projects 4, 5 and 6.

该应用程序的目的是从结构上研究聚唾液酸与各种细胞蛋白质的结合，并使用亲和力测量来表征这些相互作用。该应用一方面侧重于Siglecs（唾液酸结合受体和信号分子），另一方面侧重于CXCL 12型趋化因子。两种蛋白质类型都结合聚唾液酸，并且在两种情况下，结合与各自蛋白质的功能高度相关。在初步工作中，我们已经确定了Siglec-11蛋白中心结构域的高分辨率晶体结构，并且已经表达和纯化了各种较长的蛋白片段。在方法方面，将使用蛋白质生物化学和结构解析（NMR光谱学，X射线晶体学）以及各种亲和力测量方法。该项目与其他几个项目密切合作，最重要的是项目4、5和6。