Role of Heat Shock Proteins on Stress Response in Cyanobacteria

热激蛋白对蓝藻应激反应的作用

基本信息

  • 批准号:
    05640739
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

Heat shock proteins (HSPs) are stress proteins which are induced by various environmental stresses. Some of them are known to act as molecular chaperones which assist in the correct folding of other polypeptides and their assembly. Plants have to acclimate to the environmental change to survive and maintain themselves alive since they can not escape from the unfovarite environments. So HSPs might play important roles on the stress response in plants. Very little is known about the role of HSPs in plants on the response to the environmental stresses although a huge number of data indicate the induction of HSPs by various stresses in plants. To examine the role of heat shock proteins in adaptation to high-temperature stress in cyanobacteria, we isolated the genes for the heat shock protein from one of the transformable cyanobacteria, Synechococcus PCC7002. Analyzes of nucleotide sequences demonstrated the existence of two groEL-homologous genes in a single species of cyanobacteria. Heat shock treatment increased the levels of both groEL-homologous gene mRNAs. However, two genes were not equivalent. For example one groEL-homologous gene was accompanied by the groES gene as in E.coli groEL,while the other was not. To understand the roles of GroEL homologs in adaptation to high-temperature stress in cyanobacteria, an insertional mutant of Synechococcus PCC7002 in which one of two groEL genes was interrupted by an antibiotic resistant gene cartridge was constructed and its thermal tolerance was analyzed. Analyzes of the viability of the groEL-disrupted mutant demonstrated that both groEL gene contributed to the heat-shock response. In addition, analyzes of the thermal tolerance of photosynthetic oxygen evolution suggested that the heat shock proteins, at least one of the groEL gene did not contribute to the acquisition of the thermal tolerance of photosynthesis.
热休克蛋白(Heat shock proteins,HSPs)是由各种环境胁迫诱导产生的应激蛋白。已知它们中的一些充当分子伴侣,其有助于其它多肽的正确折叠及其组装。由于植物无法逃离无菌环境,因此必须适应环境的变化才能生存和维持生命。因此,热休克蛋白可能在植物的逆境应答中发挥重要作用。尽管大量的资料表明植物中的热休克蛋白可被各种逆境诱导产生,但对热休克蛋白在植物对逆境胁迫响应中的作用却知之甚少。为了研究热休克蛋白在蓝细菌适应高温胁迫中的作用,我们分离了热休克蛋白的基因从一个可转化的蓝细菌,聚球藻PCC 7002。核苷酸序列分析表明,存在两个groEL同源基因在一个单一的物种的蓝藻。热休克处理增加了两种groEL同源基因mRNA的水平。但是,两个基因并不等同。例如,一个groEL同源基因伴随有groES基因,如在大肠杆菌groEL中,而另一个没有。为了解蓝细菌中GroEL同源基因在高温胁迫适应中的作用,构建了一个将两个groEL基因中的一个插入抗生素抗性基因盒的聚球藻PCC 7002插入突变体,并对其耐热性进行了分析。对groEL突变体的活力分析表明,两个groEL基因都有助于热休克反应。此外,光合放氧耐热性分析表明,热休克蛋白,至少有一个groEL基因没有帮助获得光合耐热性。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nishiyama, Y., H.Hayashi, T.Watanabe and Murata: "Photosynthetic oxygen evolution is stabilized by cytochrome c550 against heat inactivation in Synechococcus sp.PCC7002" Plant Physiol.105. 1313-1319 (1994)
Nishiyama, Y., H.Hayashi, T.Watanabe 和 Murata:“细胞色素 c550 可以稳定光合产氧,以防止聚球藻 sp.PCC7002 中的热失活”Plant Physiol.105。
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    0
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Mamedov, M.D., H.Hayashi and N.Murata: "Effects of glycinebetaine and unsaturation of membrane lipids on heat stability of photosynthetic electron-transport and phosphorylation reactions in Synechocystis PCC6803." Biochim.Biophys.Acta. 1142. 1-5 (1993)
Mamedov, M.D.、H.Hayashi 和 N.Murata:“甘氨酸甜菜碱和膜脂不饱和度对集胞藻 PCC6803 光合电子传递和磷酸化反应的热稳定性的影响。”
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    0
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Mohanty, P., H.Hayashi, G.C.Papageorgiou and N.Murata: "Stabilization of the Mn-cluster of the oxygen-evolving complex by glycinebetaine." Biochim.Biophys.Acta. 1144. 92-96 (1993)
Mohanty, P.、H.Hayashi、G.C.Papageorgiou 和 N.Murata:“甘氨酸甜菜碱对放氧复合物的 Mn 簇的稳定性”。
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    0
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Nishiyama,Y.,H.Hayashi,T.Watanabe and N.Murata: "Photosynthetic oxygen evolution is stabilized by cytochrome c550 against heat inactivation in Synechococcus sp.PCC7002." Plant Physiol.105. 1313-1319 (1994)
Nishiyama,Y.,H.Hayashi,T.Watanabe 和 N.Murata:“细胞色素 c550 可以稳定光合放氧,以防止聚球藻 PCC7002 中的热失活。”
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    0
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Deshnium, P., D.A.Los, H.Hayashi, L.Mustardy and N.Murata: "Transformaiton of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress." Plant Mol.Biol.29. 897-907 (1995)
Deshnium, P., D.A.Los, H.Hayashi, L.Mustardy 和 N.Murata:“用胆碱氧化酶基因改造聚球藻可增强对盐胁迫的耐受性。”
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HAYASHI Hidenori其他文献

HAYASHI Hidenori的其他文献

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{{ truncateString('HAYASHI Hidenori', 18)}}的其他基金

Molecular Mechanism of heat-shock response and high-temperature adaptation in psychrophilic bacteria
嗜冷菌热激反应和高温适应的分子机制
  • 批准号:
    18608003
  • 财政年份:
    2006
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Phosphorylation of Proteins in Photosystem II
光系统 II 中蛋白质的磷酸化
  • 批准号:
    08044225
  • 财政年份:
    1996
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Stress Responses of Arabidopsis Transformed with the Choline Oxidase Gene
胆碱氧化酶基因转化拟南芥的胁迫反应
  • 批准号:
    08454258
  • 财政年份:
    1996
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Response to high temperature stress and enhancement of high temperature tolerance in plants studies with psychrophilic bacteria and algae
嗜冷细菌和藻类植物研究中对高温胁迫的响应和增强高温耐受性
  • 批准号:
    08554034
  • 财政年份:
    1996
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Structures and Formation Mechanisms of Folding Intermediates of Proteins
蛋白质折叠中间体的结构和形成机制
  • 批准号:
    06304051
  • 财政年份:
    1994
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
Propionic acidemia : bacterial expression system for propionyl CoA carboxylase.
丙酸血症:丙酰辅酶A羧化酶的细菌表达系统。
  • 批准号:
    06670762
  • 财政年份:
    1994
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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