Construction of Monomolecular Membrane with Different Surface Properties and Electron Transfer

不同表面性质的单分子膜的构建和电子传递

• 批准号: 05650930
• 负责人: TAKEOKA Shinji
• 金额: $ 1.34万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05650930/
• 关键词: Molecular Assembly; Surface Property; Amphiphilic Molecules; Vesicles; Molecular Orientation; Monomolecular Membrane; Hemoglobin; Electron Transfer

Monomolecular membrane having different surface properties between inner and outer surface was prepared as vesicles. The purpose of this research is to study the characteristics of this molecular assemblies and to construct functional vesicles encapsulating concentrated protein such as hemoglobin. Amphiphilic molecules of which both ends are different head groups were dispersed into water by sonication to prepare vesicles of ca.100 nm with monomolecular layr. This was confirmed from TEM observation, the measurement of the total volume of the vesicles in comparison with calculated volume, and the shift of choline methyl proton peak in ^1H-NMR by the addition of Eu^<3+> to outer aqueous phase. The resulting vesicles were very stable, and no change of vesicular size and no leakage of aqueous contents were observed. The orientation of the amphiphiles in the membrane was regulated if the size was reduced or if concentrated hemoglobin was encapsulated. Electron transfer through the membrane was analyzed from the reduction of inner oxidants by adding reductant to the outer aqueous phase.A lithocholic acid derivatives with ionic groups at both ends was incorporated to the phospholipid membrane. A significant influence of it on the molecular packing and the stability enhancement of the vesicle suspension were clarified by a fluorescence depolarization method, 1H-NMR,and a turbidity measurement.

将内表面和外表面之间具有不同表面特性的单分子膜作为囊泡制备。这项研究的目的是研究该分子组件的特征，并构建包裹浓缩蛋白（例如血红蛋白）的功能囊泡。两端的两端分子是不同的头部组，通过超声处理将其分散到水中，以制备带有单分子layr的Ca.100 nm的囊泡。这是从TEM观察结果，与计算体积相比的囊泡总体积的测量以及通过将EU ^<3+>添加到外水相中的胆碱甲基质子峰在 ^1H-NMR中的变化。所得的囊泡非常稳定，没有观察到水含量的变化，也没有观察到水含量的泄漏。如果大小降低或封装了浓缩血红蛋白，则调节膜上两亲物的方向。通过将还原剂添加到外水相的还原剂中，从氧化剂的还原剂中分析了通过膜的电子转移。将两端的离子基团岩性酸衍生物掺入磷脂膜上。通过荧光去极化方法，1H-NMR和浊度测量，阐明了它对分子填料和囊泡悬浮液的稳定性增强的显着影响。

项目成果

Z.-C.Li, F.-M.Li, S.Arase, S.Takeoka, and E.Tsuchida: ""Effect of a Lithocholic Acid Derivative on the Molecular Packing and Stability of Phospholipid Vesicles"" Chem.Lett.1994. 2199-2202 (1994)

Z.-C.Li、F.-M.Li、S.Arase、S.Takeoka 和 E.Tsuchida：“石胆酸衍生物对磷脂囊泡分子堆积和稳定性的影响”Chem.Lett。

H.Sakai, S.Takeoka, Y.Seino, and E.Tsuchida: ""Suppression of Methemoglobin Formation by Glutathione in a Concentrated Hemoglobin Solution and in a Hemoglobin-Vesicles"" Bull.Chem.Soc.Jpn.67. 1126-1129 (1994)

H.Sakai、S.Takeoka、Y.Seino 和 E.Tsuchida：“浓血红蛋白溶液和血红蛋白囊泡中谷胱甘肽对高铁血红蛋白形成的抑制”Bull.Chem.Soc.Jpn.67。

H.Sakai,S.Takeoka,Y.Seino,and E.Tsuchida: "“Suppression of Methemoglobin Formation by Glutathione in a Cocentrated Hemoglobin Solution and in a Hemoglobin-Vesicles"" Bulletin of Chemical Society of Japan. 67. 1126-1129 (1994)

H.Sakai、S.Takeoka、Y.Seino 和 E.Tsuchida：“浓缩血红蛋白溶液和血红蛋白囊泡中谷胱甘肽对高铁血红蛋白形成的抑制”，日本化学会通报 67。1126-1129。 (1994)

H.Yokohama,Y.Seino,J.E.Chung,H.Sakai,S.Takeoka,H.Nishide,and E.Tsuchida: "“Suppresion of Methemoglobin Formation in Hb-Vesicles by Glutathione"" Artificial Organs Today. 4. 107-116 (1994)

H. Yokohama、Y. Seino、J. E. Chung、H. Sakai、S. Takeoka、H. Nishide 和 E. Tsuchida：“谷胱甘肽对 Hb 囊泡中高铁血红蛋白形成的抑制”《今日人工器官》4. 107- 116 (1994)

Z.-C.Li,F.-M.Li,S.Arase,S.Takeoka,and E.Tsuchida: "“Effect of a Lithocholic Acid Derivative on the Molecular Packing and Stability of Phospholipid Vesicles"" Chemistry Letter. 1994. 2199-2202 (1994)

Z.-C.Li、F.-M.Li、S.Arase、S.Takeoka 和 E.Tsuchida：“石胆酸衍生物对磷脂囊泡分子堆积和稳定性的影响”，《化学快报》1994 年。 .2199-2202 (1994)