Construction of Monomolecular Membrane with Different Surface Properties and Electron Transfer

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Monomolecular membrane having different surface properties between inner and outer surface was prepared as vesicles. The purpose of this research is to study the characteristics of this molecular assemblies and to construct functional vesicles encapsulating concentrated protein such as hemoglobin. Amphiphilic molecules of which both ends are different head groups were dispersed into water by sonication to prepare vesicles of ca.100 nm with monomolecular layr. This was confirmed from TEM observation, the measurement of the total volume of the vesicles in comparison with calculated volume, and the shift of choline methyl proton peak in ^1H-NMR by the addition of Eu^<3+> to outer aqueous phase. The resulting vesicles were very stable, and no change of vesicular size and no leakage of aqueous contents were observed. The orientation of the amphiphiles in the membrane was regulated if the size was reduced or if concentrated hemoglobin was encapsulated. Electron transfer through the membrane was analyzed from the reduction of inner oxidants by adding reductant to the outer aqueous phase.A lithocholic acid derivatives with ionic groups at both ends was incorporated to the phospholipid membrane. A significant influence of it on the molecular packing and the stability enhancement of the vesicle suspension were clarified by a fluorescence depolarization method, 1H-NMR,and a turbidity measurement.