Comtrol mechamism of lipid metabolism helated genes by yatty acid

脂肪酸对脂质代谢相关基因的调控机制

• 批准号: 05660137
• 负责人: KAWADA Teruo
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660137/
• 关键词: Fatty acids; Gone exphessiem; Lipid metabolism; Obesity; Adipose cells; Thans criptional factor; Signal transoluction; ビタミンD受容体; レチノイン酸受容体; 脂肪酸結合タンパク質

It is important to the improvement of human health that the positive and negative regulatory systems for the signal transduction of fatty acids be well defined. Recently, fatty acids itself may act as a rigands signals to directly terget genes, and the results regulate the transcriptional control of genes on lipid metabolism and related events. In the present study, we have decided to systematically examine the effects of these fat soluble vitamins and their analogs on terminal differentiation from preadipocytes to adipocytes on the cellular and molecular aspects. The effects of RA and 1,25-OH2D3 are believed to be mediated by a specific nuclear receptor protein [retinoic acid receptor (RAR) ; designated ad subtypes alpha, beta and gamma, vitamin D receptor (VDR) ] which are members of the steroid and thyroid/retinoid receptor superfamily of ligand dependent transcriptional regulators. Furthermore, I revealed the membran asociated fatty acids receptor (mFAR) in the small intestine in rats. mFAR acts as a recognition molecule of long chain fatty acids on the signal transduction of regulatory systems for genes.

明确脂肪酸信号转导的正、负调控系统对改善人类健康具有重要意义。近年来，脂肪酸本身可能作为一种信号分子直接作用于基因，调控基因对脂质代谢及相关事件的转录调控。在本研究中，我们决定系统地研究这些脂溶性维生素及其类似物对前脂肪细胞向脂肪细胞终末分化的细胞和分子方面的影响。RA和1，25-OH 2D 3的作用被认为是由特异性核受体蛋白[视黄酸受体（RAR）;指定的ad亚型α、β和γ，维生素D受体（VDR）]介导的，其是配体依赖性转录调节因子的类固醇和甲状腺/类维生素A受体超家族的成员。此外，我还揭示了大鼠小肠的膜相关脂肪酸受体（mFAR）。mFAR作为长链脂肪酸的识别分子参与基因调控系统的信号转导。

项目成果

Yasutami.KAMEI ら: "Cloning and sequencing of the gene encoding the mouse vitamin D receptor" Gene. 152. 281-282 (1995)

Yasutami.KAMEI 等：“编码小鼠维生素 D 受体的基因的克隆和测序”Gene 152. 281-282 (1995)。

Yasutami.KAMEI ら: "The phevention of adipose differentation of 3T3-L1 cells coured by retinoic acid iselicitect through retinolc and recaptor alpha" Life Sciences. 55. PL307-312 (1994)

Yasutami.KAMEI 等人：“视黄酸诱导剂通过视黄醇和受体 α 促进 3T3-L1 细胞的脂肪分化”，生命科学 55。PL307-312 (1994)。

Yasutomi Kamei S: "Cloning and Dequencing of the gene enloding the mouse Vitamin D receptor" Gene. 152. 281-282 (1995)

Yasutomi Kamei S：“小鼠维生素 D 受体基因的克隆和测序”基因。

Y.Kamei et al.: "The prevention of adipse differentiation of 3T3-L1 cells caused by retinoic acid is elicited through retinoic acid receptor alpha." Life Sciences. 55. PL307 (1994)

Y.Kamei 等人：“由视黄酸引起的 3T3-L1 细胞脂肪分化的预防是通过视黄酸受体 α 引起的。”

Yasutomi Kamei S: "The Phevention of adipose differeihation of 3T3-L1 Cells caused by retinoic acid is elicited through vetinoic aciolreceptor alpha" Life sciences. 55. PL307-312 (1994)

Yasutomi Kamei S：“视黄酸引起的 3T3-L1 细胞脂肪分化的发生是通过兽医 aciol 受体 α 引起的”生命科学。