THE EFFECTS OF TRICHOTHECENE(T-2 TOXIN)IN THE INTESTINAL IMMUNITY AND THE SENSITIVITY TO LISTERIOSIS.

单端孢霉烯（T-2 毒素）对肠道免疫和李斯特菌敏感性的影响。

• 批准号: 05660150
• 负责人: SUGITA-KONISHI Yoshiko
• 金额: $ 1.34万
• 依托单位: NATIONAL INSTITUTE OF HEALTH OF JAPAN
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660150/
• 关键词: TRICHOTHECENE; LISTERIOSIS; IMMUNOCOMPETENT CELLS; EXPOSURE; TNF-alpha; INF-gamma; トリコテセン; 免疫担当細胞; 腸管細胞; IFN-γ; マクロファージ; T-2トキシン; Caco-2; フローサイトメトリー; T細胞; B細胞; 脾臓; 胸腺; 腸リンパ節

In the first year, the effects of trichothecene(T-2 toxin)on the immunocompetent cells were examined. The short term-exposure with middle or high dose of T-2 toxin caused the decrease of weights and the number of T cells, B cells in thymus, spleen and blood. On the hand, the long term-exposure with low does induced the change of the population of the immunocompetent cells in the lympocytes in intestine. This result suggests that even the exposure with low does of T-2 toxin has the risk of the gastrointestinal infection.In the last year, the effects of T-2 toxin on the sensitivity to listeriosis were examined. The mice exposured with middle dose of T-2 toxin showed the resistance to listeriosis though those immunocompetent cells were decreased. The production of TNF-alpha and INF-gamma in those mice rised than in no treatment mice. This result indicated the short term-exposure with trichothecene enhances the production of cytokines and then induces the acquisition the resistance to listeriosis.

在第一年，检测了二孢噻吩（T-2毒素）对免疫活性细胞的影响。中、高剂量T-2毒素短期染毒可引起小鼠体重下降，胸腺、脾脏和血液中T、B细胞数量减少。另一方面，低剂量长期暴露可引起肠道淋巴细胞中免疫活性细胞数量的变化。这一结果表明，即使是低剂量的T-2毒素暴露也有引起胃肠道感染的危险。中剂量T-2毒素染毒小鼠，免疫活性细胞减少，但仍表现出对梅毒的抵抗力。这些小鼠中TNF-α和INF-γ的产生比未治疗的小鼠增加。这一结果表明，短时间暴露于二孢噻吩可增强细胞因子的产生，从而诱导获得对二孢噻吩抗性。

项目成果

小西良子: "The effects of diefary T-2 toxin on immunocompetent cells and their functions" Proceedings of Japanese Societyof Animal Biochemisty. 30. 65-74 (1993)

Ryoko Konishi：“饲料 T-2 毒素对免疫活性细胞及其功能的影响”日本动物生物化学学会会议记录 30. 65-74 (1993)。

小西 良子: "The effects of dietary T-2 toxin on immunocompe tent cells" Proceedings of Japanese Society of Animal Biochewistry. 30. 65-74 (1993)

Ryoko Konishi：“饮食 T-2 毒素对免疫活性细胞的影响”，日本动物生物化学学会会议记录 30. 65-74 (1993)。

小西良子: "The effets of dietay T-2 toxin on immunopompetent cells and their functions." Proceedings of Japanese Society of Animal Biochemistry. 30. 65-74 (1993)

Ryoko Konishi：“饮食 T-2 毒素对免疫活性细胞及其功能的影响。”日本动物生物化学学会会议记录 30. 65-74 (1993)。

小西良子: "The cytotoxicity of Macrocyclic Trichothecenes,Roridin A and Verrucarin A on murine T-cells is reduced by Ia-negative splenic adherent cells." Toxicon. 32. 1051-1057 (1994)

Ryoko Konishi：“Ia 阴性脾贴壁细胞可降低大环单端孢霉烯族化合物、Roridin A 和 Verrucarin A 对小鼠 T 细胞的细胞毒性。” 32. 1051-1057 (1994)

小西 良子: "The cyto toxicity of Macrocy clic Tricho thecenes,Roridin A and Verrucarin A,on murine T-cells is reduced by Ia-negative splenic adnerent cells" Toxicon. 32. 1051-1057 (1994)

Ryoko Konishi：“Ia 阴性脾脏邻接细胞可降低 Macrocy clic Tricho thecenes、Roridin A 和 Verrucarin A 对小鼠 T 细胞的细胞毒性”Toxicon 32. 1051-1057 (1994)。