Electrophoretic studies on Bruguiera gymnorrhiza and Kndelia candel in Okinawa, Japan

日本冲绳木榄和木榄的电泳研究

• 批准号: 05660171
• 负责人: BABA Shigeyuki
• 金额: $ 1.28万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660171/
• 关键词: mangroves; Bruguiera gymnorrhiza; Kandelia candel; electrophoresis; allozymes; phenotypes; polyacrilamide; isozymes; 遺伝的距離; ポリアクリルアミド電気泳動

In this study, and attempt has been made to develop techiniques of the polyacrylamide gel electrophoresis (PAGE) of a vertical slab-format to analyzing isozyme loci of mangrove tree species. Two mangrove tree species were used in the study : Bruguiera gymnorrhiza and Kndelia candel in the family Rhizophoraceae. Both of two species widely are distributed in Okinawa, Japan, and distributed especially in the tropical Asian countries too. The samples of PAGE were used matured leaves of each tree collected from natural mangrove populations on Iriomote, Ishigaki, Miyako islands and Okinawa island too. Staining techniques were improved to get zymogrames clearly on the polyacrilamide gels which were consisted with 7.5% of the separation gel and 3.5% of the spacer gel. Seventeen enzyme species were stained such as acid phosphate ((ACP), eaterase (EST), gulutamate oxaloacetatate transamirase (GOT), phosphoglucomutase (PGM), peroxidase (POD), etc. Fine zymogrames were obtained 7 enzyme species out of 17 enzyme species. Phenotypes showed elecromorphs as allozymes in different individuals. Several loci of ACP,EST,PGM,GOT were estimated. All of them were either nonomers or dimers, but some of them showed null phenotypes which were not gotten exact gene frequencies. For this reason, the obtained gene frequencies were less numbers which were expected. But genetic distances are being tried to calculate.

本研究尝试建立垂直平板聚丙烯酰胺凝胶电泳（PAGE）技术，用于红树林树种同工酶位点分析。研究中使用了两种红树林树种：木榄和秋茄。这两个种都广泛分布于日本的冲绳，尤其是亚洲热带国家。PAGE分析的样品也取自西表岛、石垣岛、宫古岛和冲绳天然红树林种群的成熟叶片。改进了聚丙烯酰胺凝胶染色技术，使酶谱清晰，分离胶占7.5%，间隔胶占3.5%。对酸性磷酸酶（ACP）、酯酶（EST）、谷草转氨酶（GOT）、葡萄糖磷酸变位酶（PGM）、过氧化物酶（POD）等17种酶进行了染色，得到了7种酶的酶谱。表型显示，在不同的个体中，电变体作为等位酶。对ACP、EST、PGM、GOT等基因座进行了检测。这些基因均为非单体或二聚体，但其中部分基因表现为无效表型，尚不清楚其基因频率。因此，获得的基因频率比预期的要少。但是遗传距离正在被尝试计算。

项目成果

馬場 繁幸: "沖縄に分布するマングローブ構成樹種のアイソザイムの遺伝様式(仮題)" 南方資源利用研究会誌. (発表予定).

马场茂之：“冲绳分布的红树林树种同工酶的遗传模式（暂定标题）”南方资源利用研究会杂志（发表预定）。

馬 場 繁 幸: "沖縄に分布する二、三のマングローブ構成樹種の集団遺伝学的研究(仮題)" 第11回南資研究会研究発表会. (発表予定).

马场茂之：“冲绳分布的两种或三种红树林树种的种群遗传研究（暂定名称）”第11次南氏研究小组研究报告会（报告预定）。

馬場 繁幸: "オヒルギとメヒルギの集団遺伝学的研究(仮題)" 熱帯農業. (発表予定).

马场茂幸：《Ohirugi 和 Mehiruki 的群体遗传学研究（暂定名）》《热带农业》（待出版）。

BABA,Shigeyuki: "Fundamental studies on pollution genetics of several mangrove tree species." Jpn. J.Trop.Agr.(will be submitted).

BABA，Shigeyuki：“几种红树林树种污染遗传学的基础研究。”

馬場 繁幸: "マングローブ構成樹種の集団遺伝に関する基礎的研究(仮題)" 琉大農学報. (発表予定).

马场茂之：《红树林树种种群遗传学基础研究（暂定名）》《琉球大学农业通报》（待出版）。