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Distribution and Physiological Function of D-Amino Acids on Marine Algae

D-氨基酸在海藻中的分布及其生理功能

基本信息

批准号：
05660238
负责人：
NAGAHISA Eizo
金额：
$ 1.28万
依托单位：
School of Fisheries Sciences, Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660238/
关键词：
D-Amino Acid D-Aspartate D-Alanine D-Amino Acid Oxidase D-Aspartate Oxidase Alga Physiological function Racemase ヒジキ

项目摘要

I.The biologically rare D-aspartate was found in extracts of marine macroalgae. DL-Aspartate was isolated from the Phaeophyta, Hizikia fusiformis, By ion-exchange chromatography, and crystallized from aqueous ethanol. It was characterized by optical-rotatory-dispersion analysis and reversed phase HPLC analysis. The crystal consisted of equal amounts of D and I isomers. The presence of free D-aspartate was same method (HPLC) . Generally, Phaeophyta contained a high concentration of D-aspartate in contrast with the low concentration in Chlorophyta and Rhodophyta. Particularly in Costaria costata, Hizikia fusiformis and Sargassum yezoensis, belonging to those of L-aspartate.II.D-Aspartate content in marine alga, Hizikia fusiformis, was determined in various seasons and different parts of the alga. D-Aspartate content varied seasonally ; it was high from late autumn to early spring and low in summer. The content also differed among various parts of the alga ; it was generally high in the adhesive root and main branch, and that in the blade was less than half that in the adhesive root. The content in the receptacle which was obtained from August sample was showed a level similar to that in the adhesive root or the main branch. From these results, we have assumed that free D-aspartate might play important roles in the early stage of growth of Hizikia fusiformis.III.Occurrence of free D-alanine was verified in various marine macroalgae by the reversedphase HPLC analysis. The presence of free D-alanine was detected in 15species (54%) out of the total of 24 species of algae examined. The content was high in Hizikia fusiformis, Heterochordaria abietina, Sargassum nigrifolium of Phaeophyta (96,83 and 78nmol/g wet wt., respectively) . The ratio of D/L was different among algae species (0.03-0.49) , and there was no correlation between D-alanine content and D/L ratio.

在海洋大型藻类的提取物中发现了生物学上罕见的D-天冬氨酸。从褐藻中分离得到DL-天冬氨酸。通过旋光色散分析和反相HPLC分析对其进行了表征。晶体由等量的D和I异构体组成。游离D-天冬氨酸的存在采用相同的方法（HPLC）。褐藻门中D-天冬氨酸含量较高，而绿藻门和红藻门中D-天冬氨酸含量较低。特别是Costaria costata、Hizikia fusiformis和Sargassum yezoensis，属于L-天冬氨酸的种类。II.测定了海洋鱼类Hizikia fusiformis在不同季节和不同部位的D-天冬氨酸含量。D-天门冬氨酸含量随季节变化，秋末至早春高，夏季低。不同部位的含量也有差异，一般在粘着根和主分支中含量较高，叶片中含量不到粘着根的一半。8月份采集的花托中的含量与主根或主分支中的含量相近。从这些结果，我们已经假设，游离D-天冬氨酸可能发挥重要作用的早期阶段的生长Hizikia fusiformis.III。发生游离D-丙氨酸被证实在各种海洋大型藻类的反相HPLC分析。在所检测的24种藻类中，有15种（54%）检出了游离D-丙氨酸。褐藻门的羊栖菜、冷杉异索藻、黑叶马尾藻中含量较高（分别为96、83和78 nmol/g湿重），分别）。不同藻种的D/L值不同（0.03-0.49），D-丙氨酸含量与D/L值无相关性。