Experimental analysis on organ allergy of hapten-bound renal tubular basement membrane

将三硝基苯酚偶联的卵清蛋白高度阳离子化(等电点为10)，经凝胶过滤得到42 kDa的单体。将该抗原选择性注入Wistar大鼠左肾动脉。冰冻切片免疫组织化学染色证实，抗原定位于肾小管上皮细胞基底膜和包膜上，呈线状沉积在肾小球基底膜上，而在肾小球基底膜上未见分布，注射抗原后36h仍可见。对照组，非阳离子单体TNP-OA(42 kDa，等电点=4.6)在肾小管上皮细胞内可见细小颗粒状沉积，但在肾小管上皮细胞上无明显颗粒沉积。皮下注射阳离子抗原7天后致敏大鼠急性肾小管坏死，使大鼠产生TNP特异性细胞免疫。该病变与人过敏性肾炎的病变不同，可能与TNP毒性有关。在先前注射阳离子抗原的非致敏C57/BL6小鼠中，针对TNP的脾T细胞(无论是CD4细胞还是CD8细胞)的转移不有助于TIN的诱导。

Trinitrophenol-conjugated ovalbumin was highly cationized (isoelectric point>10) and was subjected to gel filtration to obtain monomer (42 kDa). The antigen was injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen section ; it was deposited in a liner pattern on the tubular basement membrane (TBM) and Bowman's capsule but not on the glomerular basement membrane, and remained up to 36 h after the antigen injection. In control, non-cationized, monomeric TNP-OA (42 kDa, isoelectric point=4.6) showed fine granular deposition in the tubular epithelium exclusivery, but not on the TBM.Tubulointerstitial nephritis (TIN) with neutrophilic infiltration was induced by the serum transfer of antiTNP-human IgG rabbit serum 24 h after cationic antigen injection. Acute tubular necrosis was induced after cationic antigen injection in the rats which were 7 days-previously skin-sensitized by picryl chloride to have TNP-specific cellular immunity. The lesion was not similar to the lesion of human hypersensitivity nephritis and may due to the TNP-toxicity. The transfer of splenic T cell (either CD4+ or CD8+) against TNP did not contribute to TIN-induction in the non-sensitized C57/BL6 mice which were previously injected with the cationic antigen.