STIMULATING ACTION OF MULTIPLE VIRULENCE FACTORS PRODUCED BY Vibrio cholerae non-O1

非 O1 霍乱弧菌产生的多种毒力因子的刺激作用

• 批准号: 05670255
• 负责人: YOH Myonsun
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670255/
• 关键词: NAG vibrio; enterotoxin; E1 Tor hemolysim; choleta toxin; thermostable direct hemolysim; protease; O139; heat stable enterotoxin (ST)

Vibrio cholerae strains other than serovar O1 (non-O1 V.cholerae) have been known to cause diarrhea. Gastroenteritis associated with V.cholerae non-O1 shows vareous clinical symptomes, such as mucous and bloody diarrhea, vomiting, nausea, fever, abdominal pain as well as watery diarrhea similar to cholera due to V.cholerae O1. Novel serogroup V.cholerae O139 have caused epidemics in India or Bangladesh since 1992. Severe clinical sympotme due to O139 were undistinguishable from cholerae due to V.cholerae O1. These various symptomes may be explained by the production of a varaiety of toxins by V.cholerae non-O1, including cholera toxin (CT) -like toxin, thermostable direct hemolysin (TDH) -like toxin (NAG-rTDH), heat-stable (ST) -like toxin (NAG-ST), El Tor hemolysin-like toxin, and hemagglutinin/protease (Vc-HA/P). We already purified toxins and analyzed biological activity of each toxins. As multiple virulence factors are seemed to affect each other in vivo actually, we analyzed stimulating action of multiple pathogenic factors as well as identification of virulence factors produced by O139.The results we have got in this periods are (1) we showed unstability of ctchi gene in chromosomal gene of O139 by using restriction fragment pattem, (2) we purified protease produced by O139 and compared to protease of V.cholerae O1, showing both proteases are undistinguishable, (3) Protease treatment of rabbit ileal loop before challenge of E1 Tor-hemolysin-like toxin showed increased fluid accumulation activity because E1 Tor hemolysin-like toxin is known to be processed by protease produced by V.cholerae non-O1 and results in increasing of activity, (4) we observed stimulating action between ct-like enterotoxinand NAG-ST,NAG-ST and E1 Tor hemolysin-like toxin in suckling mouse assay.

已知O 1血清型以外的霍乱弧菌菌株（非O 1霍乱弧菌）可引起腹泻。与非O 1型霍乱弧菌相关的胃肠炎表现出各种临床症状，如粘液性和血性腹泻、呕吐、恶心、发热、腹痛以及水样腹泻，类似于由O 1型霍乱弧菌引起的霍乱。自1992年以来，新型血清群霍乱弧菌O 139在印度或孟加拉国引起流行病。由O 139引起的严重临床症状与由V. cholesterol O 1引起的cholesterol无法区分。这些不同的毒素可以通过非O 1霍乱弧菌产生各种毒素来解释，包括霍乱毒素（CT）样毒素、热稳定直接溶血素（TDH）样毒素（NAG-rTDH）、热稳定（ST）样毒素（NAG-ST）、El Tor溶血素样毒素和血凝素/蛋白酶（Vc-HA/P）。我们已经纯化了毒素并分析了每种毒素的生物活性。由于多种毒力因子在体内实际上似乎是相互影响的，我们分析了多种致病因子的刺激作用，并对O 139产生的毒力因子进行了鉴定。（2）对O 139菌株产生的蛋白酶进行了纯化，并与霍乱弧菌O 1菌株的蛋白酶进行了比较，结果表明两种蛋白酶不能区分，（3）在E1 Tor-溶血素样毒素攻击前，蛋白酶处理兔回肠袢显示出增加的液体积聚活性，因为E1 Tor-溶血素样毒素-（4）在乳鼠实验中观察到ct-样肠毒素与NAG-ST、NAG-ST和E1 Tor溶血素样毒素之间的刺激作用。

项目成果

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于明顺：《生物危害物质的管理与运输》清洁技术。3. 27-31 (1993)。

Nagayama,K.et al.: "Correlation between cell-associated mannose-sensitive hemagglutination by V.parahaemolyticus and adherence to a human colonic" FEMS Microbiology Letters. 120. 207-210 (1994)

Nagayama,K.等人：“副溶血弧菌引起的细胞相关甘露糖敏感血凝作用与人类结肠粘附之间的相关性”FEMS 微生物学快报。

野田 孝治: "海外旅行者から分離されたCT産生性古典ウ-ボン型エルトールコレラ菌。" 臨床検査. 37. 1155-1157 (1993)

Koji Noda：“从海外旅行者中分离出产生 CT 的经典乌汶型埃尔托霍乱。” 37. 1155-1157 (1993)。

MYONSUN YOH: "Development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene." J.Clin.Microbiol.31. 1312-1314 (1993)

MYONSUN YOH：“开发霍乱毒素基因的酶标记寡核苷酸探针。”

Yoshio Ichinose et al.: "The effects on enterotoxicity of protease purified from Vibrio cholerae O1." FEMS Microbiol.Lett.115. 265-272 (1994)

Yoshio Ichinose 等人：“从霍乱弧菌 O1 中纯化的蛋白酶对肠毒性的影响。”