Research on biochemical analysis of mechanism of hemolysis induced by TDH produced by Vibrio parahaemolyticus.

点击翻译按钮获取中文摘要

We performed this study to make clear the mechanism of hemolysis induced by Vibrio parahaemolyticus thermostable direct hemolysin (TDH). Following results were obtained.(1) TDH bound to erythrocytes both of which sensitive and insensitive to TDH in similar affinity. Binding of TDH to erythrocytes depends on temperature.(2) TDH induced phosphorylation of 25 kDa protein on TDH-sensitive erythrocyte membrane but not on TDH-insensitive erythrocyte membrane.(3) Phosphorylation of 25 kDa protein was inhibited by protein kinase C inhibitors. Hemolytic activity of TDH was also inhibited by protein kinase C inhibitors.(4) Mutant TDH,R7 which could bind to erythrocytes but lost hemolytic activity, could not induce phosphorylation of 25 kDa protein.These results suggest that the phosphorylation of 25 kDa protein on erythrocyte membrane is essential to lead erythrocytes lysis.