Research on biochemical analysis of mechanism of hemolysis induced by TDH produced by Vibrio parahaemolyticus.

副溶血弧菌产生TDH引起溶血机制的生化分析研究

• 批准号: 07670309
• 负责人: YOH Myonsun
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670309/
• 关键词: Vibrio parahaemolyticus; TDH; Biochemical study; Pathogenesis; Hemolysis; Phosphorylation

We performed this study to make clear the mechanism of hemolysis induced by Vibrio parahaemolyticus thermostable direct hemolysin (TDH). Following results were obtained.(1) TDH bound to erythrocytes both of which sensitive and insensitive to TDH in similar affinity. Binding of TDH to erythrocytes depends on temperature.(2) TDH induced phosphorylation of 25 kDa protein on TDH-sensitive erythrocyte membrane but not on TDH-insensitive erythrocyte membrane.(3) Phosphorylation of 25 kDa protein was inhibited by protein kinase C inhibitors. Hemolytic activity of TDH was also inhibited by protein kinase C inhibitors.(4) Mutant TDH,R7 which could bind to erythrocytes but lost hemolytic activity, could not induce phosphorylation of 25 kDa protein.These results suggest that the phosphorylation of 25 kDa protein on erythrocyte membrane is essential to lead erythrocytes lysis.

我们进行这项研究是为了明确副溶血弧菌耐热直接溶血素（TDH）引起溶血的机制。得到如下结果：(1)TDH与对TDH敏感和不敏感的红细胞结合，亲和力相似。 TDH与红细胞的结合取决于温度。(2)TDH诱导TDH敏感的红细胞膜上25kDa蛋白的磷酸化，但不诱导TDH不敏感的红细胞膜上的25kDa蛋白的磷酸化。(3)蛋白激酶C抑制剂抑制25kDa蛋白的磷酸化。蛋白激酶C抑制剂也能抑制TDH的溶血活性。(4)突变型TDH,R7能与红细胞结合但失去溶血活性,不能诱导25 kDa蛋白磷酸化。这些结果表明红细胞膜上25 kDa蛋白的磷酸化对于导致红细胞裂解至关重要。

项目成果

余 明順: "腸炎ビブリオ感染症" 化学療法の領域. 12. 633-640 (1996)

于明顺：《副溶血性弧菌感染》化疗领域12。633-640（1996）。

Yoh, M., N.Morinaga, M.Noda, and T.Honda: "The binding of Vibrio parahaemolyticus ^<125>I-labeled thermostable direct hemolysin to erythrocytes" Toxicon.33. 651-657 (1995)

Yoh，M.，N.Morinaga，M.Noda，和T.Honda：“副溶血弧菌^ 125 I标记的热稳定直接溶血素与红细胞的结合”Toxicon.33。

Yoh, M., N.Kawakami, Y.Funakoshi, K.Okada, and T.Honda: "Evaluation of two assay kits for thermostable direct hemolysin (TDH) as an indicator of TDH-related hemolysin (TRH) produced by Vibrio parahaemolyticus" Microbiol.Immunol.39. 157-159 (1995)

Yoh, M.、N.Kawakami、Y.Funakoshi、K.Okada 和 T.Honda：“作为副溶血弧菌产生的 TDH 相关溶血素 (TRH) 指标的热稳定性直接溶血素 (TDH) 的两种检测试剂盒的评估

Yoh, M., G.Q,Tang, T.Iida, N.Morinaga, M.Noda, and T.Honda: "Phosphorylation of a 25kDa protein is induced by thermostable direct hemolysin of Vibrio parahaemolyticus" Int.J.Biochem.Cell.Biol.23. 1365-1369 (1996)

Yoh, M.、G.Q、Tang、T.Iida、N.Morinaga、M.Noda 和 T.Honda：“副溶血弧菌的热稳定性直接溶血素诱导 25kDa 蛋白的磷酸化”Int.J.Biochem.Cell。

Tang, G.Q., T.Iida, K.Yamamoto, and T.Honda: "Ca^<2+>-independent cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin (TDH) on intestine 407, a cell line derived from human embryonic intestine" FEMS Micobiol.Lett.134. 233-238 (1995)

Tang、G.Q.、T.Iida、K.Yamamoto 和 T.Honda：“副溶血性弧菌热稳定性直接溶血素 (TDH) 对肠 407（一种源自人胚胎肠的细胞系）的 Ca^2 独立细胞毒性”FEMS Micobiol