Biochemical studies on the relationship between Clostridium perfringens alpha toxin and CTP-binding protein in rabbit erythrocytes.

产气荚膜梭菌α毒素与兔红细胞CTP结合蛋白关系的生化研究。

• 批准号: 05670271
• 负责人: SAKURAI Jun
• 金额: $ 1.41万
• 依托单位: Tokushima Bunri University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670271/
• 关键词: Clostridium perfringens; alpha Toxin; Phospholipase C; Phospholipase D; Phospholipid metabolism; Site-directed-mutagenesis; Histidine; Hemolysis; ウェルシュ菌; ガス壊疽; alpha毒素; GTP-結合タンパク質; ホスファチジル4,5-二リン酸; ホスファチジン酸; プロテインキナーゼC

We replaced all nine histidine residues in Clostridium perfringens alpha toxin with neutral amino acids (alanine, leucine and glycine) by site-direct mutagenesis and investigated the relationship between the biological activities and histidine residues or zinc ion in the toxin.The results suggested that the histidine-11 residue in the toxin is regarded as an essential and integral part of the toxin protein, that the histidine-68, -126 and 136 residues bind an exchangeable and labile divalent metal which is important for binding to membranes and substrates and that the histidine-148 residue tightly binds one zinc metal which is essential for the active or catalytic site of alpha toxin.

我们用中性氨基酸替换了产气荚膜梭菌α毒素中的所有9个组氨酸残基结果表明，组氨酸-11残基是该毒素蛋白质的重要组成部分，组氨酸-68残基是该毒素蛋白质的重要组成部分，组氨酸-68残基是该毒素蛋白质的重要组成部分，组氨酸-68残基是该毒素蛋白质的重要组成部分。组氨酸-126和136残基结合可交换的和不稳定的二价金属，其对于结合膜和底物是重要的，组氨酸-148残基紧密结合一种锌金属，其对于α毒素的活性或催化位点是必需的。

项目成果

Jun Sakurai, Sadayuki Ochi and Hiroshi Tanaka.: "Evidence for coupling of Clostridium perfringens alpha-toxin-induced hemolysis to stimulated phosphatidic acid formation in rabbit erythrocytes." Infect.Immun.61(9). 3711-3718 (1993)

Jun Sakurai、Sadayuki Ochi 和 Hiroshi Tanaka.：“产气荚膜梭菌 α 毒素诱导的溶血与刺激兔红细胞中磷脂酸形成耦合的证据。”

M. Nagahama, H, Lida, E. Nishioka,K.ikemoto and J. Sakurai: "Roles of the carboxy-terminal region of Clostridium perfringens alpha toxin." FEMS Microbiol.Lett.120. 297-302 (1994)

M. Nagahama、H、Lida、E. Nishioka、K.ikemoto 和 J. Sakurai：“产气荚膜梭菌 α 毒素羧基末端区域的作用”。

M. Nagahama, H. Iida and J. Sakurai: "Effect of clostridium perfringens epsilon toxin on rat isolated aorta." Microfiol. Immunol.37. 447-450 (1993)

M. Nagahama、H. Iida 和 J. Sakurai：“产气荚膜梭菌 epsilon 毒​​素对大鼠离体主动脉的影响”。

J. Sakurai, S-Ochi and H. Tanaka: "Evidence for coupling of clostridium perfringens alpha-toxin-induced hemolysis to stimulated phosplatidic acid formation in rabbit erythrocytes" Infect.Immun.61. 3711-3718 (1993)

J. Sakurai、S-Ochi 和 H. Tanaka：“产气荚膜梭菌 α 毒素诱导的溶血与兔红细胞中刺激的磷脂酸形成耦合的证据”Infect.Immun.61。

櫻井 純: "イラストレイテッド微生物学" 南山堂, 350 (1993)

樱井淳：《微生物学图解》Nanzando，350（1993）