Biochemical and pharmacological studies on Clostridium perfringens alpha toxin

产气荚膜梭菌α毒素的生化和药理研究

• 批准号: 62570198
• 负责人: SAKURAI Jun
• 金额: $ 1.41万
• 依托单位: Tokushima Bunri University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570198/
• 关键词: Clostridium perfringens; Alpha toxin; Ileum; Contraction; Ca channel; Protein kinase C; プロテインキ ナーゼC; アラキドン酸; プロテインキナーゼC; ホスファチジルイノシトール; ホスファチジン酸; トロンボキサンA_2

We have recently demonstrated that alpha toxin-induced contraction of rat isolated aorta was associated with phosphatidyl inositol metabolism and Ca uptake and that the toxin contracted the rat isolated ileum. The present study was undertaken to define the mode action of the contractile effect on smooth muscle tissues. It was suggested that the contractile response of rat isolated aorta to the toxin is associated with stimulation of thromboxane A_2 production from arachidonic acid released by the toxin in the endothelial cells of the tissue. On the other hand, the toxin induced phosphatidyl inositol metabolism and Ca uptake in the rat isolated ileum. However, it caused no stimulation of arachidonic acid release and malondialdehyde production in the tissue. Therefore, it appears that the initial action of the toxin is stimulation of phosphatidyl inositol metabolism and Ca uptake.

我们最近证明了α毒素诱导的大鼠离体主动脉收缩与磷脂酰肌醇代谢和钙摄取有关，毒素收缩了大鼠离体回肠。本研究旨在确定收缩效应对平滑肌组织的模式作用。提示大鼠离体主动脉对毒素的收缩反应与毒素释放的花生四烯酸刺激组织内皮细胞产生血栓素A_2有关。另一方面，毒素诱导大鼠离体回肠磷脂酰肌醇代谢和钙摄取。然而，它没有引起组织中花生四烯酸释放和丙二醛产生的刺激。因此，毒素的初始作用似乎是刺激磷脂酰肌醇代谢和钙摄取。

项目成果

Jun Sakurai; Yoshio Fujii; Kunimasa Torii; Keiko Kobayashi: "Dissociation of various biological activities of Clostridium perfringens alpha toxin by chemical modification." Toxicon. 27. 317-323 (1989)

樱井淳；

櫻井純: "医学細菌学4巻:Clostridium perfri ngensの毒素" 菜根出版, 315-364 (1989)

樱井淳：《医学细菌学第 4 卷：产气荚膜梭菌毒素》Nane Publishing，315-364 (1989)

Jun Sakurai and Yoshio Fujii: Journal of Pharmacology & Experimental therapseatics.

Jun Sakurai 和 Yoshio Fujii：药理学杂志

Jun Sakurai: Toxicon.

樱井润：Toxicon。

Yoshio Fujii; Jun Sakurai: "Contraction of the rat isolated aorta caused by Clostridium perfringens alpha toxin (phospholipase C): evidence for the involvement of arachidonic acid metabolism." Br. J. Pharmacol.In press. (1989)

藤井芳夫；