Identification of HTLV-1 neutralization epiotpe amino acid sequence its and application

HTLV-1中和表位氨基酸序列的鉴定及其应用

• 批准号: 05670284
• 负责人: TANAKA Yuetsu
• 金额: $ 1.34万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670284/
• 关键词: HTLV-I; Neutralization; Epiotpe; Reptide; Vaccine; アミノ酸配列; 単クロン抗体

In order to define neutralization regions the envelope antigen of human T-cell leukemia virus type-I (HTLV-I) we have generated a number new antienvelope gp46 mAbs fromrats and mice. Epitopes recognized by new mAbs, which could neutralize HTLV-I in syncytium and transformation inhibition assays, were localized to gp46 amino acid sequences, 186-193,190-195,191-195,191-196 and 194-199. Ovalbumin (OVA) -conjugated synthetic gp46 peptides containing these neutralization epitopes, amino acids 190-199 (pep190-199) and pep180-204, but not pep185-194 or pep194-203, could give rise to HTLV-I neutralizing antibody responses in rabbits. These immune or non-immune rabbits were then challenged with HTLV-I by i. v. inoculation with 5x10^7 live HTLV-I producer ILT-8M2 cells. By a polymerase chain reaction (PCR) assay, it was revealed that HTLV-I provirus was detected in PBL from non-immune and pep288-312-immunized rabbits, whereas the provirus was not detected in PBL from the prp190-199 and pep180-204-immunized rabbits over an extended period. These results suggest that the induction of anti-gp46 neutralizing antibody responses by immunization with synthetic peptides has the potential to protect animals against HTLV-I infection in vivo.

为了确定人T细胞白血病病毒Ⅰ型（HTLV-Ⅰ）包膜抗原的中和区，我们从大鼠和小鼠中制备了一系列新的抗包膜gp 46单克隆抗体。在合胞体和转化抑制试验中，新mAb识别的可中和HTLV-1的表位定位于gp 46氨基酸序列186- 193、190 - 195、191 - 195、191 -196和194-199。含有这些中和表位的卵清蛋白（OVA）缀合的合成gp 46肽（氨基酸190-199（pep 190 -199）和pep 180 -204，但不含pep 185 -194或pep 194 -203）可在兔中引起HTLV-I中和抗体应答。这些免疫或非免疫兔然后用HTLV-I通过i. v.接种5x10^7活HTLV-I生产者ILT-8 M2细胞。通过聚合酶链反应（PCR）分析，揭示了在来自非免疫和pep 288 -312免疫的兔的PBL中检测到HTLV-I前病毒，而在来自prp 190 -199和pep 180 -204免疫的兔的PBL中在较长时间内未检测到前病毒。这些结果表明，通过用合成肽免疫诱导抗gp 46中和抗体应答具有在体内保护动物免受HTLV-I感染的潜力。

项目成果

田中勇悦: "Induction of amtibody response that neutralize human T-cell leukemia virus type I in fection typeI in vitco and in vivo by peptide immunization" Journal of Virology. 68. 6323-6331 (1994)

Yuetsu Tanaka：“通过肽免疫在体外和体内诱导 I 型感染中和人 T 细胞白血病病毒 I 型的抗体反应”，《病毒学杂志》68. 6323-6331 (1994)。

田中勇悦ら,他8名: "Induction of antibody response that neutralize human T-cell leukemia virus type I infection in vitro and in vivo by peptide immunization" Journal of Virology. 68. 6323-6331 (1994)

Yuetsu Tanaka 等人和其他 8 人：“通过肽免疫在体外和体内诱导中和人 T 细胞白血病病毒 I 型感染的抗体反应”Journal of Virology 68. 6323-6331 (1994)。

Tanaka, Y etal: "Induction of antibody responses that neutralize human T-cell leukemia virus type I infection in vitro and in vivo by peptide immunization" J,Virol. 68 Oct. 6323-6331 (1994)

Tanaka, Y 等人：“通过肽免疫在体外和体内诱导中和人 T 细胞白血病病毒 I 型感染的抗体反应”J，Virol。