Analysis of human foamy virus (HFV) as a putative pathogen for autoimmune diseases

人类泡沫病毒（HFV）作为自身免疫性疾病推定病原体的分析

• 批准号: 05670432
• 负责人: TAMURA Naoaki
• 金额: $ 1.34万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670432/
• 关键词: Human Foamy Virus; familial Mediterranean fever; bel-2; PCR; Human foamy virus; プロウイルス; PCR(ポリメラーゼ連鎖反応); 自己免疫疾患; 逆転写酵素活性

We report here the evidence of Human Foamy Virus (HFV) infection in patients with familial Mediterranean fever (FMF) in Japan. Although HFV is the first reported human retrovirus, no definite relation to a specific disease has been reported. Then, we hypothesized that HFV might be one of putative pathogens for some of autoimmune diseases and surveyed to detect HFV gene elements in 320 patients with several diseases including IPF,sarcoidosis, SLE,RA,cancer and 3 cases of FMF.The presence of HFV gene elements in genomic DNA was evaluated by the PCR amplification followed by Southern blot hybridization with HFV cDNA clone. Doing so, existence of HFV gene element was observed in all patients of FMF.None of other patients showed positive signals of HFV provirus. FMF is a disease of unknown etiology characterized with symptoms such as periodic fever, chest pain, abdominal pain and arthralgia. Although FMF is considered to be an inherited disease, there are many FMF patients without having clear genetic background. Our result revealed that a subgroup of FMF in Japan has already been infected with HFV.Because we have very few patients of FMF in Japan, it is difficult to get clear conclusion. But we think that our result could partially support the idea that HFV takes part in the development of FMF which cannot be explained by an inherited fashion alone.

我们在此报告日本家族性地中海热(FMF)患者感染人类泡沫病毒(HFV)的证据。虽然HFV是第一个报告的人类逆转录病毒，但尚未有明确的与特定疾病的关系的报道。然后，我们假设HFV可能是某些自身免疫性疾病的病原体之一，并对320例包括IPF、结节病、SLE、RA、癌症和3例FMF在内的几种疾病进行了HFV基因元件的检测。所有FMF患者均存在HFV基因元件，其他患者均未见HFV前病毒阳性信号。FMF是一种病因不明的疾病，以周期性发热、胸痛、腹痛和关节痛为特征。虽然FMF被认为是一种遗传性疾病，但有许多FMF患者没有明确的遗传背景。我们的结果显示，日本的FMF亚群已经感染了HFV，由于日本FMF患者很少，很难得出明确的结论。但我们认为，我们的结果可能部分支持HFV参与FMF发展的观点，这不能仅用遗传方式来解释。

项目成果

田村尚亮: "間質性肺疾患と内在性レトロウイルス" 最新医学. 49. 307-312 (1994)

Naoaki Tamura：“间质性肺疾病和内源性逆转录病毒”现代医学 49. 307-312 (1994)。