LC-MS-QTOF-System

LC-MS-QTOF系统

• 批准号: 432736281
• 金额: --
• 依托单位: Universität Trier
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/432736281?language=en
• 关键词: LC; MS; QTOF; System

Several departments of the faculty VI „Regional and Environmental Sciences“ of the University Trier apply for a system for high-resolution liquid chromatography-mass spectrometry (LC-MS-QTOF). The system will (i) replace an older liquid chromatography-tandem mass spectrometry-system (in use since 2006) and (ii) provide new options for more selective and sensitive analytics, which especially enables screening analysis of complex mixtures of substances of anthropogenic as well as natural origin. The need for such analytics is justified by on-going and planned projects. Being the only analytical system of this kind at the University Trier it makes a powerful enrichment for the analytical equipment and will be cooperatively used within the group of departments and laboratory cluster, respectively, as is the case with the existing equipment. Environmental analysis using mass spectrometry is done in research and education since many years in the department of Soil Science (applicant) as well as in the co-applying departments and further departments of the faculty VI. The aforementioned liquid chromatography-tandem mass spectrometry-system provided very good analytical options at date of purchase that substantially fostered the acquisition of various funded projects. Due to its service life, the system is no more reliable; downtimes and repair times increased to an extent that conflicts with routine operation and use in time-limited projects.Therefore, we apply for a liquid chromatography-mass spectrometry-system that is capable for high-resolution determination of unknown compounds and can also be used for routine detection and quantification of known compounds („non-target“ and „target“ analysis). This proposal is, thus, aimed not only for a replacement purchase but at the same time combined with a significant innovation, offering new analytical options. This is especially justified by the high-resolution analysis that is recently not possible and allows to simultaneously detect and identify (at least by the sum formula) large numbers of compounds. This enables to realize innovative studies on substance identification: Determination and identification of compound mixtures, identification of metabolites and degradation pathways, high-resolution non-target analysis. The substantially higher sensitivity allows to reach limits of detection that are by magnitudes lower compared to the existing system. This will be achieved in particular in complex matrices that are especially relevant in environmental sciences (e.g., extracts of plants and animal tissues, souls and sediments, waste water, sludge and wastes). The much broader mass range offers new application options, perspectively up to the analysis of proteins (Proteomics). The required efforts for operation and maintenance as well as the expected running costs correspond to the performance framework of the applying departments.

特里尔大学VI“区域和环境科学”学院的几个部门申请了一个高分辨率液相色谱-质谱（LC-MS-QTOF）系统。该系统将㈠取代较旧的液相色谱-串联质谱系统（自2006年以来一直在使用），㈡为更具选择性和灵敏度的分析提供新的选择，特别是能够对人为和自然来源的物质的复杂混合物进行筛选分析。正在进行的和计划中的项目证明了这种分析的必要性。作为特里尔大学唯一的这种分析系统，它极大地丰富了分析设备，并将分别在部门和实验室集群内协同使用，就像现有设备一样。多年来，在土壤科学系（申请人）以及共同申请部门和学院VI的其他部门，使用质谱法进行环境分析。上述液相色谱-串联质谱系统在购买之日提供了非常好的分析选择，大大促进了各种资助项目的获得。由于其使用寿命，系统不再可靠，停机时间和维修时间增加到与常规操作和在有限时间的项目中使用相冲突的程度。因此，我们申请了一种液相色谱-质谱系统，该系统能够高分辨率测定未知化合物，也可以用于常规检测和定量已知化合物（“非目标”和“目标”分析）。因此，这一建议不仅是为了替代购买，同时也是一项重大创新，提供了新的分析选择。这是特别合理的高分辨率分析，最近是不可能的，并允许同时检测和识别（至少通过总和公式）大量的化合物。这使得能够实现物质识别的创新研究：化合物混合物的测定和识别、代谢物和降解途径的识别、高分辨率非目标分析。显著更高的灵敏度允许达到与现有系统相比低幅度的检测极限。这将特别在与环境科学特别相关的复杂基质中实现（例如，植物和动物组织的提取物、灵魂和沉积物、废水、污泥和废物）。更广泛的质量范围提供了新的应用选择，尤其是蛋白质分析（蛋白质组学）。运营和维护所需的努力以及预期的运行成本与应用部门的绩效框架相对应。