Ultra-high performance liquid chromatograph coupled to a time-of-flight mass spectrometer with ion mobility separation (UHPLC/IMS-QTOF-MS)

超高性能液相色谱仪与具有离子淌度分离功能的飞行时间质谱仪联用 (UHPLC/IMS-QTOF-MS)

• 批准号: 427540127
• 金额: --
• 依托单位: Hochschule Geisenheim University
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/427540127?language=en
• 关键词: Ultra; performance; liquid; chromatograph; coupled

Briefly after the foundation of Geisenheim University in 2013, the German Council of Science and Humanities has recommended the formation and strengthening of research-oriented professorships as well as the meanwhile established right for independently conducting doctorates. Thus, Geisenheim University now consequently supports the application of the newly appointed research professor, Prof. Dr. habil. Ralf Schweiggert, for funding of an Ultra-High-Performance Liquid Chromatography (UHPLC) system coupled to an ion mobility separation (IMS) device and a time-of-flight mass analyzer (TOF-MS).Currently, UHPLC/IMS-TOF-MS systems as well as high resolution mass spectrometers are unavailable at Geisenheim University. The requested system will allow the comprehensive and state-of-the-art characterization of technologically-, physiologically-, and regulatorily-relevant phytochemicals, residues, and contaminants in plant-based raw materials, foods, beverages, and processing by-products. Besides enabling high resolution mass determinations to determine the sum formula of molecular and fragment ions, the requested system allows the ion mobility-based separation of otherwise unresolvable isobaric and often co-eluting compounds. We expect to gain substantially deeper insights into the composition of plant materials, particularly foods, and their changes during post-harvest treatments, processing and storage. Thereby, the system will not only contribute to the development, optimization and analyses of novel and traditional foods in the future, but will also allow targeting legally relevant research questions such as the query for the origin of a food or that for elucidating which technology has been applied during food production.A decentralized management and utilization concept regarding the requested system is targeted, since the analytical competence to run the requested system is - at Geisenheim University - only available in the applicant’s workgroup. An analytical core facility is inexistent and not desired by Geisenheim University. Externalization of high resolution MS work has been tested and proven to be unsuitable for the research goals of the applicant. The requested system would not only allow the applicant’s workgroup to step up to the state-of-the-art and beyond, but would also ideally fit the analytical needs for the workgroup's research goals, being perfectly complementary to existing methods well established at the workgroup and Geisenheim University. Ultimately, the equipment would allow numerous new and innovative research opportunities along the whole food chain.

盖森海姆大学于2013年成立后不久，德国科学与人文理事会建议成立和加强研究型教授职位，同时确立独立教授学位的权利。因此，盖森海姆大学现在支持新任命的研究教授哈比勒教授的申请。Ralf Schweiggert，资助与离子迁移率分离(IMS)设备和飞行时间质谱仪(TOF-MS)相耦合的超高性能液相色谱(UHPLC)系统。目前，盖森海姆大学无法提供UHPLC/IMS-TOF-MS系统和高分辨率质谱仪。所要求的系统将允许对植物性原材料、食品、饮料和加工副产品中的技术、生理和法规相关的植物化学物质、残留物和污染物进行全面和最先进的表征。除了实现高分辨率质量测定以确定分子离子和碎片离子的总式外，所要求的系统还允许基于离子迁移率的分离，否则无法分解的等压化合物和通常是共洗脱的化合物。我们希望对植物材料的组成，特别是食品，以及它们在收获后处理、加工和储存过程中的变化有更深入的了解。因此，该系统不仅将有助于未来新型和传统食品的开发、优化和分析，而且还将允许针对法律上相关的研究问题，如查询食品来源或阐明在食品生产过程中应用了哪些技术。关于所请求的系统的分散管理和利用的概念是有针对性的，因为运行所请求的系统的分析能力-在盖森海姆大学-只有在申请人的工作组中才能获得。分析核心设施是不存在的，也不是盖森海姆大学想要的。高分辨率MS工作的外部化已被测试和证明不适合申请者的研究目标。所要求的系统不仅将允许申请人的工作组达到最先进的水平和更高的水平，而且还将理想地满足工作组研究目标的分析需求，与工作组和盖森海姆大学建立的现有方法完美互补。最终，这些设备将在整个食物链中提供无数新的和创新的研究机会。