Research of tissue remodelling in chronic obstructive pulmonary diseases

慢性阻塞性肺疾病组织重塑研究

基本信息

  • 批准号:
    05670514
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

1. With in situ hybridization, mRNA's of TNFalpha, TGFalpha, TGFbeta1 and PDGF-B were detected in bronchial epithelial cells, fibroblasts and macrophages of bronchial tissues from patients with chronic bronchitis, and mainly in eosinophils of those from asthmatics. In contrast, in control subjects, there were few cells expressing TNFalpha and PDGF-B mRNA,although a few cells were localized with TGFbeta1 mRNA.2. Semi-quantative analysis of the density of cells expressing these mRNA's showed : (1) the cell density only for PDGF-B mRNA in bronchial asthma was significantly geater than that in normal control, (2) the density for TNFalpha and TGFbeta1 mRNA in chronic bronchitis was greater than that in either asthma or normal control, and (3) the density for PDGF-B mRNA in chronic bronchitis was as great as that in asthma. These results suggest that the pattern of growth factors expressed at the site of inflammation is important to develop the specific pathophysiological condition in chronic inflammatory airway diseases such as chronic bronchitis and bronchial asthma.3. With immunohistochemistry, immunoreactive TNFalpha and PDGF-B protein was detected in bronchial epithelial cells, endothelial cells, fibroblasts and macrophages of bronchial tissues from patients with chronic bronchitis and asthma, but not in bronchial tissues from normal subjects.4. Immunoreactive IL-5, IL-8 and GM-CSF protein was detected in sputum cells from patients with bronchial asthma and chronic bronchitis. The percentage of cells positive for IL-5 and GM-CSF in asthma was greater than that in chronic bronchitis, and the percentage for IL-8 in chronic bronchitis was greater than that in asthma. These results clearly indicate that the profile of cytokine expression detected in sputum cells reflects pathological features in each disease, suggesting the usefulness of sputum for analyzing cellular and molecular events in chronic airway inflammation.
1.用原位杂交法检测了慢性支气管炎患者支气管上皮细胞、成纤维细胞和巨噬细胞中TNF α、TGF α、TGF β 1和PDGF-B的mRNA,哮喘患者支气管组织中以嗜酸性粒细胞为主。相反,在对照组中,有很少的细胞表达TNF α和PDGF-B mRNA,尽管有少数细胞定位于TGF β 1 mRNA。表达这些mRNA的细胞密度的半定量分析显示:(1)支气管哮喘组仅PDGF-BmRNA表达的细胞密度明显高于正常对照组;(2)慢性支气管炎组TNF α和TGF β 1 mRNA表达的细胞密度均高于哮喘组和正常对照组;(3)慢性支气管炎和哮喘患者PDGF-B mRNA表达密度相同。这些结果表明,生长因子在炎症部位的表达模式对于慢性炎症性气道疾病如慢性支气管炎和支气管哮喘的特定病理生理状态的发展是重要的.免疫组化检测结果显示,慢性支气管炎和哮喘患者支气管组织中的上皮细胞、内皮细胞、成纤维细胞和巨噬细胞表达TNF α和PDGF-B蛋白,而正常人支气管组织中未见TNF α和PDGF-B蛋白.用免疫组化法检测支气管哮喘和慢性支气管炎患者痰细胞中IL-5、IL-8和GM-CSF蛋白。哮喘组IL-5、GM-CSF阳性细胞百分率高于慢性支气管炎组,慢性支气管炎组IL-8阳性细胞百分率高于哮喘组。这些结果清楚地表明,痰细胞中检测到的细胞因子表达谱反映了每种疾病的病理特征,表明痰用于分析慢性气道炎症中的细胞和分子事件的有用性。

项目成果

期刊论文数量(48)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kouhei Yamauchi: Chronic airway inflammation and adhesion molecules. In Allergic inflammatory diseases. Y.Miyaji and M.Kurosawa, editors.Sentan-igakusya, 199-207 (1994)
Kouhei Yamauchi:慢性气道炎症和粘附分子。
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
Isao Chno: "Expression of growth factors by eosinophils in asthmatic bronchial tissues" J.Clin.Invest.(発表予定).
Isao Chno:“哮喘支气管组织中嗜酸性粒细胞的生长因子表达”J.Clin.Invest.(待提交)。
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    0
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  • 通讯作者:
Isao Ohno: Ishiyaku-syuppan Co.Ltd.Platelet-derived growth factor.In Respiratory diseaases- state of arts.S.Kitamura and M.Harasawa, editors., 83-85 (1994)
Isao Ohno:Ishiyaku-syuppan Co.Ltd.血小板衍生生长因子。呼吸系统疾病 - 艺术现状。S.Kitamura 和 M.Harasawa,编辑。,83-85 (1994)
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  • 影响因子:
    0
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Yuichi Ohkawara: "In situ expression of the cell adhesion molecules in bronchial tissues from asthmatics with air flow limitation iin vivo evidence of VCAM-1/VLA-4---" Am.J.Respir.Cell Mol.Biol.12. 4-12 (1995)
Yuichi Ohkawara:“气流受限的哮喘患者支气管组织中细胞粘附分子的原位表达,VCAM-1/VLA-4 的体内证据——”Am.J.Respir.Cell Mol.Biol.12。
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  • 影响因子:
    0
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大野 勲: "粘膜上皮と粘膜炎症-気管支喘息をモデルとして" 免疫薬理. 11. 270-277 (1993)
Isao Ohno:“粘膜上皮和粘膜炎症 - 使用支气管哮喘作为模型”免疫药理学 11. 270-277 (1993)。
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    0
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OHNO Isao其他文献

Sex-related functional differences in CD4+ T cells and CD8+ T cells mediate female-predominant inflammation in allergic asthma
CD4 T 细胞和 CD8 T 细胞的性别相关功能差异介导过敏性哮喘中女性为主的炎症
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    MIYASAKA Tomomitsu;ITO Chihiro;DOBASHI-OKUYAMA Kaori;SATO Miki;MASUDA Chiaki;KAWANO Tasuku;OHKAWARA Yuichi;KIKUCHI Toshiaki;TAKAYANAGI Motoaki;OHNO Isao
  • 通讯作者:
    OHNO Isao
ブロンコレアとはどんな疾患でしょうか?
支气管炎是一种什么样的疾病?
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    OUCHI Ryusuke;KAWANO Tasuku;MIYASAKA Tomomitsu,OHKAWARA Yuichi;TAKAYANAGI;Motoaki;TAKAHASHITomoko;OHNO Isao;鈴川真穂.
  • 通讯作者:
    鈴川真穂.
The increased susceptibility to allergic asthma with the impairment of respiratory tolerance by psychological stress
心理压力导致呼吸耐受力受损,对过敏性哮喘的易感性增加
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
  • 作者:
    ISHIGAKI Takahiro;KAWANO Tasuku;MIYASAKA Tomomitsu;OHKAWARA Yuichi;TAKAYANAGI Motoaki;OHNO Isao
  • 通讯作者:
    OHNO Isao
Early life stress increased the risk of adult onset asthma through the inhibition of the development of respiratory tolerance in murine model
早期生活压力通过抑制小鼠模型呼吸耐受的发展而增加成年哮喘的风险
  • DOI:
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    0
  • 作者:
    OUCHI Ryusuke;KAWANO Tasuku;MIYASAKA Tomomitsu,OHKAWARA Yuichi;TAKAYANAGI;Motoaki;TAKAHASHITomoko;OHNO Isao
  • 通讯作者:
    OHNO Isao
Psychological stress increases susceptibility to the development of asthma through inhibiting respiratory tolerance
心理压力通过抑制呼吸耐受性增加患哮喘的易感性
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KAWANO Tasuku;ISHIGAKI Takahiro;Nitta Norihide;MIYASAKA Tomomitsu;OHKAWARA Yuichi;TAKAYANAGI Motoaki;OHNO Isao
  • 通讯作者:
    OHNO Isao

OHNO Isao的其他文献

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{{ truncateString('OHNO Isao', 18)}}的其他基金

Role of functional single-nucleotide polymorphism of the opioid receptor gene in stress-induced exacerbation of asthma
阿片受体基因功能性单核苷酸多态性在应激诱发哮喘恶化中的作用
  • 批准号:
    25461164
  • 财政年份:
    2013
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of opioid receptors-allergic immune responses axis in stress-induced asthma
阿片受体-过敏性免疫反应轴在应激性哮喘中的作用
  • 批准号:
    22590843
  • 财政年份:
    2010
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of opioid network in asthma exacerbations evoked by psychological stress
阿片类药物网络在心理压力引起的哮喘发作中的作用
  • 批准号:
    19590909
  • 财政年份:
    2007
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The study on the regulation of allergic responses by opioid network in bronchial asthma
阿片网络调节支气管哮喘过敏反应的研究
  • 批准号:
    16616007
  • 财政年份:
    2004
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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嗜酸性粒细胞在细菌感染过程中的作用
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通过针对肥大细胞和嗜酸性粒细胞的主调节因子来治疗感染和炎症性疾病
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嗜酸性粒细胞在 SIV 感染中的作用
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    10669240
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Are there different types of eosinophils?
嗜酸性粒细胞有不同类型吗?
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Eosinophils facilitate bacterial clearance by mediating antibacterial T cell responses in the respiratory tract
嗜酸性粒细胞通过介导呼吸道中的抗菌 T 细胞反应来促进细菌清除
  • 批准号:
    10090774
  • 财政年份:
    2021
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Eosinophils facilitate bacterial clearance by mediating antibacterial T cell responses in the respiratory tract
嗜酸性粒细胞通过介导呼吸道中的抗菌 T 细胞反应来促进细菌清除
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Eosinophils facilitate bacterial clearance by mediating antibacterial T cell responses in the respiratory tract
嗜酸性粒细胞通过介导呼吸道中的抗菌 T 细胞反应来促进细菌清除
  • 批准号:
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Role of ILC2 and eosinophils in abdominal aortic aneurysm
ILC2 和嗜酸性粒细胞在腹主动脉瘤中的作用
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  • 财政年份:
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