Research of tissue remodelling in chronic obstructive pulmonary diseases

1.用原位杂交法检测了慢性支气管炎患者支气管上皮细胞、成纤维细胞和巨噬细胞中TNF α、TGF α、TGF β 1和PDGF-B的mRNA，哮喘患者支气管组织中以嗜酸性粒细胞为主。相反，在对照组中，有很少的细胞表达TNF α和PDGF-B mRNA，尽管有少数细胞定位于TGF β 1 mRNA。表达这些mRNA的细胞密度的半定量分析显示：（1）支气管哮喘组仅PDGF-BmRNA表达的细胞密度明显高于正常对照组;（2）慢性支气管炎组TNF α和TGF β 1 mRNA表达的细胞密度均高于哮喘组和正常对照组;（3）慢性支气管炎和哮喘患者PDGF-B mRNA表达密度相同。这些结果表明，生长因子在炎症部位的表达模式对于慢性炎症性气道疾病如慢性支气管炎和支气管哮喘的特定病理生理状态的发展是重要的.免疫组化检测结果显示，慢性支气管炎和哮喘患者支气管组织中的上皮细胞、内皮细胞、成纤维细胞和巨噬细胞表达TNF α和PDGF-B蛋白，而正常人支气管组织中未见TNF α和PDGF-B蛋白.用免疫组化法检测支气管哮喘和慢性支气管炎患者痰细胞中IL-5、IL-8和GM-CSF蛋白。哮喘组IL-5、GM-CSF阳性细胞百分率高于慢性支气管炎组，慢性支气管炎组IL-8阳性细胞百分率高于哮喘组。这些结果清楚地表明，痰细胞中检测到的细胞因子表达谱反映了每种疾病的病理特征，表明痰用于分析慢性气道炎症中的细胞和分子事件的有用性。

1. With in situ hybridization, mRNA's of TNFalpha, TGFalpha, TGFbeta1 and PDGF-B were detected in bronchial epithelial cells, fibroblasts and macrophages of bronchial tissues from patients with chronic bronchitis, and mainly in eosinophils of those from asthmatics. In contrast, in control subjects, there were few cells expressing TNFalpha and PDGF-B mRNA,although a few cells were localized with TGFbeta1 mRNA.2. Semi-quantative analysis of the density of cells expressing these mRNA's showed : (1) the cell density only for PDGF-B mRNA in bronchial asthma was significantly geater than that in normal control, (2) the density for TNFalpha and TGFbeta1 mRNA in chronic bronchitis was greater than that in either asthma or normal control, and (3) the density for PDGF-B mRNA in chronic bronchitis was as great as that in asthma. These results suggest that the pattern of growth factors expressed at the site of inflammation is important to develop the specific pathophysiological condition in chronic inflammatory airway diseases such as chronic bronchitis and bronchial asthma.3. With immunohistochemistry, immunoreactive TNFalpha and PDGF-B protein was detected in bronchial epithelial cells, endothelial cells, fibroblasts and macrophages of bronchial tissues from patients with chronic bronchitis and asthma, but not in bronchial tissues from normal subjects.4. Immunoreactive IL-5, IL-8 and GM-CSF protein was detected in sputum cells from patients with bronchial asthma and chronic bronchitis. The percentage of cells positive for IL-5 and GM-CSF in asthma was greater than that in chronic bronchitis, and the percentage for IL-8 in chronic bronchitis was greater than that in asthma. These results clearly indicate that the profile of cytokine expression detected in sputum cells reflects pathological features in each disease, suggesting the usefulness of sputum for analyzing cellular and molecular events in chronic airway inflammation.