The physiological role of posterior pituitary alpha-melanocyte stimulating hormone in ultraviolet radiation-induced skin pigmentation.

垂体后叶α-黑素细胞刺激激素在紫外线辐射引起的皮肤色素沉着中的生理作用。

• 批准号: 05670748
• 负责人: MURAI Ichiro
• 金额: $ 1.41万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670748/
• 关键词: skin pigmentation; the posterior pituitary; ultraviolet; alpha-melanocyte stimulating hormone; tyrosinase; hairless mouse; bromocriptine; 皮膚色素沈着; αMSH; alphaMSH

Using ultraviolet (UV) -sensitive male hairless mouse ; (HR-1xHR/De) F1, this study was conducted to investigate the physiological role of posterior pituitary alpha-melanocyte stimulating hormone (alphaMSH) in UV radiation (UVR) -induced skin pigmentation.The results of in vivo and in vitro experiments are as follows : (1) either posterior pituitary lobectomy (LOBEX) or hypophysectomy reduced plasma alphaMSH levels to approximately 20% of control (SHAM ; sham operation) values and completely abolished UV-induced skin pigmentation, (2) administration of alphaMSH to these groups restored skin response to UVRs with normalized plasma alphaMSH levels, (3) epidermal tyrosinase activity (TA ; a rate limiting enzyme for melanin synthesis) showed a positive-correlation to plasma alphaMSH levels, (4) TA in SHAM,LOBEX + alphaMSH and LOBEX groups increased remarkably following UVRs, although TA in LOBEX mice was only 20% of SHAM group, (5) plasma alphaMSH levels did not alter following a single or … More multiple UVR, (6) treatment of bromocriptine (BROM ; a dopamine agonist), similarly to LOBEX,decreased plasma alphaMSH levels but increased basal TA significantly compared to that in SHAM group, (7) BROM-treated mice also showed skin pigmentation by UVRs with similarly increased post-UVRs TA to SHAM, (8) dopamine inhibited alphaMSH secretion in a dose-dependent manner from the cultured posterior pituitary cells, and (9) alphaMSH,BROM and UVR increased TA in cultured skin tissue, however co-incubation with 10^<-7> M of neither alphaMSH nor BROM potentiated stimulatory effects of UVR on TA.These data suggest that circulating alphaMSH is derived mainly from the posterior pituitary and participates in UVRs-induced skin pigmentation through epidermal basal TA.However, it is likely that unidentified factors and / or mechanisms may induce skin pigmentation following UVR rather than mediation by circulating alphaMSH,since UVR did not alter alphaMSH secretion from the posterior pituitary and had stimulatory effects on TA in the cultured skin tissues under alphaMSH free condition. Less

使用紫外线（UV）敏感的雄性无毛小鼠;（HR-1xHR/De）F1，本研究旨在探讨垂体后叶α-黑素细胞刺激素的生理作用体内和体外实验的结果如下：（1）垂体后叶切除术（LOBEX）或垂体切除术均使血浆α MSH水平降至对照组的约20%，（2）向这些组施用α MSH恢复了皮肤对UVRs的反应，血浆α MSH水平正常化，（3）表皮酪氨酸酶活性降低，（4）SHAM、LOBEX + alphaMSH和LOBEX组的TA在UVRs后显著增加，尽管LOBEX小鼠的TA仅为SHAM组的20%;（5）单次或多次照射后血浆alphaMSH水平没有改变， ...更多信息 多次紫外线照射，（6）溴隐亭治疗（BROM ;多巴胺激动剂）与LOBEX类似，降低血浆α MSH水平，但与SHAM组相比显著增加基础TA，（7）BROM处理的小鼠也显示出通过UV R的皮肤色素沉着，与SHAM类似地增加UV R后TA，（8）多巴胺以剂量依赖性方式抑制培养的垂体后叶细胞的α MSH分泌，（9）α-MSH、BROM和UVR均可增加培养皮肤组织中TA的含量，但与10 μ M α-MSH或BROM共孵育均不能增强UVR对TA的刺激作用。这些数据表明，循环中的α-MSH主要来自垂体后叶，并通过表皮基底TA参与UVR诱导的皮肤色素沉着。然而<-7>，可能是未鉴定的因素和/或机制可能在UVR后诱导皮肤色素沉着，而不是通过循环α MSH介导，由于UVR不改变垂体后叶分泌α-MSH，并且在无α-MSH的培养皮肤组织中对TA具有刺激作用，条件少

项目成果

村井一郎: "皮膚黒化機構における下垂体後葉性α-melanocyte-stimolating hormoneの役割" 日大医学雑誌. 54(5). 261-267 (1995)

Ichiro Murai：“垂体后叶 α-黑素细胞刺激激素在皮肤变黑机制中的作用”日本大学医学杂志 54(5) (1995)。

Magara, M., Murai, I.and Nakagawa, S.: "The role of the posterior pituitary in ultraviolet radiation-induced skin pigmentation." J.Nihon Univ.Med.Ass.53(11). 872-878 (1994)

Magara, M.、Murai, I. 和 Nakakawa, S.：“垂体后叶在紫外线辐射引起的皮肤色素沉着中的作用。”

村井一郎: "皮膚黒化機構における下垂体後葉性α-melanocyte-stimulating hormoneの役割" 日大医学雑誌. 54. 261-267 (1995)

Ichiro Murai：“垂体后叶 α-黑素细胞刺激激素在皮肤变黑机制中的作用”日本大学医学杂志 54. 261-267 (1995)。

真柄綱夫、村井一郎、中川滋木: "紫外線照射によるhairless mouseの皮膚黒化機構における下垂体後葉の役割" 日大医学雑誌. 53. 872-878 (1994)

Tsunao Magara、Ichiro Murai、Shigeki Nakakawa：“垂体后叶在紫外线照射引起的无毛小鼠皮肤变黑机制中的作用”日本大学医学杂志 53. 872-878 (1994)。

Murai, I.: "The physiological role of posterior pituitary alpha-melanocyte stimulating hormone in ultraviolet radiation-induced skin pigmentation" J.Nihon Univ.Med.Ass.54(5). 261-267 (1995)

Murai, I.：“垂体后叶 α-黑素细胞刺激激素在紫外线辐射引起的皮肤色素沉着中的生理作用”J.Nihon Univ.Med.Ass.54(5)。