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Studies on the Expression and the Regulation of Activin in Human Pituitary and Pituitary Adenoma Tissues.

人垂体和垂体腺瘤组织中激活素表达和调控的研究。

基本信息

批准号：
05670882
负责人：
DEMURA Reiko
金额：
$ 1.47万
依托单位：
Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Activins have been known to modulate various pituitary hormone secretion. To study the localization and the regulation of activins in human pituitary, we studied the expression of inhibin alpha, beta_A, beta_B subunit mRNAs and activin type II receptor mRNA in various human pituitary tissues. We found that inhibin alpha and beta_A subunits and activin type II receptor mRNAs were expressed, with the ubiquitous presence of beta_A subunit mRNA in all human pituitary adenoma tissues, regardless of their hormone production. We further measured individual mRNA levels by a quantitative RT-PCR.The inhibin alpha subunit mRNA levels were undetectably low in all 9 cultured adenoma tissues, but beta_A mRNA levels were 0.3830<plus-minus>0.071 in 3 GH adenomas, 0.672<plus-minus>0.140 in 3 prolactinomas and 0.957<plus-minus>0.414 molecules/cell in 3 non-functioning adenomas.The addition of 10^<-8>M activin A decreased the beta_A mRNA level within 4 hours in 5 out of 9 adenomas, though the decreases were not statistically significant. Furthermore, activinimmunoreactivities were demonstrated in the cytoplasm of some of the adenomas, while inhibin alpha subunit immunoreactivity was negative in all of the tissues studied. The data demonstrated a local synthesis of activin in pituitary adenoma tissues in preference to inhibin and its local role in human pituitary adenoma tissues.

已知激活素调节各种垂体激素分泌。为了研究激活素在人垂体中的定位和调节，我们研究了垂体素α、β_A、β_B亚基mRNA和激活素II型受体mRNA在人垂体不同组织中的表达。我们发现垂体腺瘤组织中均有垂体素α和β_A亚基及激活素Ⅱ型受体mRNA的表达，且β_A亚基mRNA在所有垂体腺瘤组织中均存在，而与其激素分泌无关。9例垂体腺瘤组织中β_A mRNA水平均较低，而3例GH腺瘤中β_A mRNA水平为0.3830 ± 0.071，3例泌乳素腺瘤中β_A mRNA水平为<plus-minus>0.672 <plus-minus>± 0.140，3例<plus-minus>无功能腺瘤中β_A mRNA水平为0.957 ± 0.414分子/细胞，10 μ <-8>M激活素A可使其中5例垂体腺瘤中β_A mRNA水平在4小时内下降，但无统计学意义。此外，在一些腺瘤的细胞质中显示了活性素免疫反应，而在所有研究的组织中，α-受体结合素α亚基免疫反应均为阴性。这些数据表明，激活素在垂体腺瘤组织中的局部合成优先于白藜芦醇及其在人垂体腺瘤组织中的局部作用。