Preoperative assessment for malignant behavior of upper gastrointestinal tract

桥粒是细胞间的连接，在上皮细胞黏附中起着重要的作用。我们在电子显微镜研究中发现，在食道癌细胞中桥粒的数量减少。桥粒糖蛋白I(DGI)是桥粒膜核心的主要组成部分，在上皮细胞黏附中起重要作用。正常鳞状上皮细胞在其细胞-细胞交界处强烈表达DGI。根据DGI表达的强度和方式，将癌组织分为三组：DGI(++)、DGI(+)和DGI(-)。DGI(+)或DGI(-)肿瘤的淋巴结转移率高于DGI(++)。DGI的表达与肿瘤侵袭、淋巴结转移、淋巴管侵袭、血管侵袭呈负相关。DGI在转移性淋巴结中的表达强度与原发灶相同或较低。因此，DGI表达的降低或缺失可能促进淋巴结转移。Thoromomodul…更多的(TM)是胸腺肽受体，最初在内皮细胞上发现，起到天然的抗凝血剂的作用。TM免疫组织化学染色主要表达于鳞状上皮，主要位于细胞间桥。癌组织中TM主要表达于细胞-细胞交界处和胞浆。比较原发灶和转移灶中TM的表达，发现转移灶中TM的表达远低于原发灶。TM的表达下调可能在胃癌的转移过程中起重要作用，在胃癌中，DGI的表达下调与淋巴结转移、淋巴侵袭和淋巴结转移数目有关。与C-erb B2、P53、FH6和PCNA相比，DGI的表达与淋巴结转移密切相关，且HLADR阳性细胞或多个S-100蛋白阳性树突状细胞阳性患者的生存率明显高于其他患者，这些结果可能为上消化道恶性行为提供一些重要信息。较少

Desmosomes are intercellular junctions which play an important role in epithelial cell adhesion. We reported a decrease in number of desmosomes in esophageal cancer cells as shown by electron microscopic study. Desmoglein I (DGI) is a major component of the desmosomal membrane core that plays an important role in epitherial cell adhesion. Normal squamous epitherial cells strongly expressed DGI at their cell-cell boundaries. According to the intensity and pattern of DGI expression, the cancerous tissues were divided into three groups : DGI (++), DGI (+), and DGI (-). DGI (+) or DGI (-) tumors had lymph node metastases more frequently than DGI (++). A good inverse correlation between DGI expression and tumor invasion, lymph node metastasis, lymphatic invasion and vessel invasion was observed. DGI expression in metastatic lymph nodes was the same or of less intensity, than in the primary tumor. Therefore, reduction or loss of DGI expression may promote lymph node metastases. Thorombomodul … More in (TM) is thorombin receptor that identified originally on the endothelium and acts a natural anticoagulant. TM was immunohistochemically expressed in the squamous epithelium mainly at the intercellular bridges. The carcinoma tissues expressed TM mainly at the cell-cell boundaries and in the cytoplasm. When TM expression was compared between the primary and metastatic lesions, TM expression was far fewer in the metastatic lesions than in the primary tumors. The reduction of TM expression seems to play an important role in the metastatic process.In the patinets with gastric carcinoma, a down-regulation of DGI expression and lymph node metastasis, lymphatic invasion and the number of lymph node metastasis. A close relationship between DGI expression and lymph node metastasis was found, compared with C-erbB2, p53, FH6 and PCNA.Furthermore, survival rate of patients with HLA-DR positive cells or with many S-100 protein positive dendritic cells was significantly better than that of the other patients.These results may provide us with some important infomations for malignant behavior of the upper gastrointestinal tract. Less