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Photodynamic effects using a diode laser with mono-L-aspartyl Chlorin e6 for implanted mice tumor

使用单-L-天冬氨酰二氢叶酸 e6 的二极管激光器对植入小鼠肿瘤的光动力效应

基本信息

批准号：
05671135
负责人：
OKUNAKA Tetsuya
金额：
$ 1.09万
依托单位：
Tokyo Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Photodynamic therapy (PDT) using the photosensitizer drug Photofrin has recently been approved for the treatment of early stage lung cancer, esophageal cancer, gastric cancer, uterine cancer and uterine dysplasia by the Japanese Ministry of Health and Welfare. The authors performed photodynamic therapy, avording any hyperthermic effects, using a diode laser and another photosensitizer mono-L-aspartyl chlorin e6 (NPe6) in an implanted fibrosarcoma in mice and achieved tumor therapeutic effects. The tissue temperature rise was 3.8ﾟC when 100mW/cm^2 light was delivered, this temperature being below the temperature range of hyperthermia. In the present study, PDT was performed 5 hours following the administration of 5.0 mg/kg NPe6. With 50,100,150 and 200 J/cm^2, tumor cure rates were 20,50,70 and 90%, respectively. In addition, when PDT was performed with administration of 1.25,2.5,5.0,7.5 and 10.0 mg/kg NPe6 with 100 J/cm^2 laser exposure, tumor cure rates were 0,20,50,70 and 90%, respectively. In some arimals, tumor recurrence seemed to occur from deeper layr of tumors. Therefore, the relationship between the effects of PDT and residual amounts of NPe6 in the deeper level of tumors following the PDT procedure were examined. We used a CCD camera system to measure the fluorescence intensity to evaluate the residual amount of NPe6 at 3mm from the tumor surface. The ratio of the residual amount of NPe6 following 50,100,150 and 200J/cm^2 laser exposure to no laser exposure were 73.4,36.0,22.0 and 15.9% respectively. These results suggest that a certain increase in the tumor tissue level of NPe6 and a certain increase of laser light dose reaching deeper layr of tumor caused an increase in percent cure. In addition, the effectiveness of PDT and also tumor recurrence following PDT depend to some extent on the total laser laser dose reaching deeper layrs of tumors. Furthermore, the effectiveness of PDT tends to correlate with the amount of photobleaching by PDT.

使用光敏剂药物Photofrin的光动力疗法（PDT）最近已被日本厚生省批准用于治疗早期肺癌、食道癌、胃癌、子宫癌和子宫发育不良。作者采用半导体激光和另一种光敏剂单-L-乙酰二氢卟酚e6（NPe 6）对小鼠移植性纤维肉瘤进行光动力学治疗，获得了肿瘤治疗效果。当提供100 mW/cm ^2的光时，组织温升为3.8 ℃，该温度低于热疗的温度范围。在本研究中，在施用5.0 mg/kg NPe 6后5小时进行PDT。在50、100、150和200 J/cm ^2时，肿瘤治愈率分别为20、50、70和90%。另外，在100 J/cm ^2激光照射下，以1.25、2.5、5.0、7.5和10.0 mg/kg NPe 6进行PDT时，肿瘤治愈率分别为0、20、50、70和90%。在某些病例中，肿瘤复发似乎发生在肿瘤的深层。因此，检查PDT的效果与PDT程序后肿瘤更深层次中NPe 6残留量之间的关系。我们使用CCD摄像系统测量荧光强度，以评估距肿瘤表面3 mm处NPe 6的残留量。50、100、150和200 J/cm ^2激光照射后NPe 6的残留量分别为对照组的73.4%、36.0%、22.0%和15.9%。这些结果表明，肿瘤组织中NPe 6水平的一定增加和到达肿瘤深层的激光光剂量的一定增加引起治愈百分比的增加。此外，PDT的有效性以及PDT后的肿瘤复发在一定程度上取决于到达肿瘤更深层的总激光剂量。此外，PDT的有效性往往与PDT的光漂白量相关。