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Mechanism of ischemic tolerance phenomenon in hippocampal CAI sector of the gerbil

沙鼠海马CAI区缺血耐受现象的机制

基本信息

批准号：
05671185
负责人：
NAKAGOMI Tadayoshi
金额：
$ 1.34万
依托单位：
Teikyo University School of Med.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Following brief cerebral ischemia, tolerance to subsequent ischemia is induced in the hippocampal neurons. The aim of this study was to investigate the relationship between ischemic tolerance phenomenon and protein synthesis. First, recovery of protein synthesis was studied autoradiographically in gerbils with induced tolerance. To observe the effect of tolerance acquisition, double forebrain ischemia, 2-min ischemia followed by 5 min-ischemia, was induced 2 days later. In this group, recovery of protein synthesis in the CAI sector was rapid. At 1 day of recirculation, protein synthesis returned near normal. On the other hand, protein synthesis in the 5-min ischemia group was severely suppressed and never returned to the normal level. The present study revealed an early recovery of protein synthesis in the hippocampal neurons in the gerbil with induced tolerance. Secondly, possible differences in electrophoresis pattern of the brain protein between tolerance-induce animals and animals injected with protein synthesis inhibitor. There was no differences in the pattern of the protein between these two groups. Thirdly, we investigated whether multiple sublethal ishemia can enhance the ability of tolerance induction or prolong the period of tolerance. At 1 month after the multiple exposure to sublethal 2-min ischemia, animals did not acquire ischemic tolerance to 4-min lethal ischemia. To clarify the mechanism of ischemic tolerance, further studies are needed.

短暂的脑缺血后，海马神经元对随后的缺血产生耐受。本研究旨在探讨缺血耐受现象与蛋白质合成的关系。首先，恢复蛋白质的合成进行了研究放射自显影在沙鼠诱导耐受。为观察耐受获得的效果，在2天后诱导双前脑缺血，缺血2min，再缺血5min。在该组中，CAI区蛋白质合成的恢复很快。在再循环的第1天，蛋白质合成恢复接近正常。另一方面，5分钟缺血组的蛋白质合成受到严重抑制，从未恢复到正常水平。本研究揭示了早期恢复的蛋白质合成在海马神经元中诱导耐受的沙鼠。第二，耐受诱导动物和蛋白质合成抑制剂注射动物脑蛋白质电泳图谱的可能差异。这两组之间的蛋白质模式没有差异。第三，探讨了多次亚致死性缺血是否能增强耐受诱导能力或延长耐受时间。在多次暴露于亚致死性2分钟缺血后1个月，动物没有获得对4分钟致死性缺血的缺血耐受。缺血耐受的机制有待进一步研究。