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Immunological effect of CB-11mAb on intervertebral disc degeneration

CB-11mAb对椎间盘退变的免疫学作用

基本信息

批准号：
05671207
负责人：
TSUJI Haruo
金额：
$ 1.22万
依托单位：
TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671207/
关键词：
Monoclonal antibody Intervertebral disc Immunology Immunohistochemistry

项目摘要

Type II collagen existing in the hyaline cartilage and nucleus pulposus of the intervertebral disc have a specific property as an isolated antigen for immunological reactions.In considering the mechanism of intervertebral disc degeneration, annular rupture may provide contact with Type II collagen in the nucleus pulposus and the lymphocytes in vivo. In humans, not a few patients show severe multiple intervertebral disc degeneration irrespective of their clonological ages, suggesting an immulogical background to represent disc degeneration. From the experimental results of hyaline cartilage observation, CB-11 peptide of Type II collagen is a specific fragment of immunoreactivity of T II C.In this reserch project, mamy efforts have been paid to refine the specific monoclonal antibody of CB-11 peptide (CB-11mAb). The pilot study clarified that the CB-11mAb recognized the antigen epitope of T II C from the results of immunosuppressive efects of CB-11mAb on collagen-induced arthritis in mice. Using an ABC method, serial immunohistochemical sections from 12 fresh intervertebral discs (mainly nucleus pulposus with various grade of degeneration) obtained by surgery was reacted with the CB-11mAb. However, none of positive immunoreactivity of Type II collagen was observed in degenerated nucleus pulposus.Thus the conclusion was drawn to be negligible on T II C immunologic mechanism on the process of intervertebral disc degeneration in humans.

存在于椎间盘的透明软骨和髓核中的II型胶原具有作为免疫反应的分离抗原的特异性，考虑到椎间盘退变的机制，环破裂可能提供与髓核中的II型胶原和体内淋巴细胞的接触。在人类中，无论其克隆学年龄如何，不少患者表现出严重的多发性椎间盘退变，这表明椎间盘退变的免疫学背景。从透明软骨观察的实验结果来看，Ⅱ型胶原CB-11肽是T Ⅱ C免疫反应性的特异性片段。本研究旨在制备CB-11肽的特异性单克隆抗体（CB-11 mAb）。初步研究表明CB-11 mAb对小鼠胶原诱导性关节炎的免疫抑制作用表明CB-11 mAb识别T Ⅱ C抗原表位。采用ABC法，对12个新鲜椎间盘（主要是不同程度退变的髓核）进行免疫组织化学染色。而退变髓核中未见Ⅱ型胶原免疫反应阳性，因此，TII-C免疫机制在人类椎间盘退变过程中的作用是不可忽视的。