Studies on vaccine therapy for rheumatoid arthritis.

类风湿性关节炎疫苗治疗的研究。

• 批准号: 05671212
• 负责人: INOUE Koji
• 金额: $ 1.41万
• 依托单位: SHIGA UNIVERSITY OF MEDICAL SCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671212/
• 关键词: type II collagen; rheumatoid arthritis; T cell receptor; estrogen receptor; synovium; chondrocyte; hybridoma; T cell recptor; T細胞; 慢性関節リウマチ; II型コラーゲン; T細胞受容体; ワクチン; II型コラーゲン(typeII collagen); T cell; clone

A type II collagen (CII) reactive T cell clone (JT1) was established by IL-2 stimulation. This reactivity to CII was shown to be MHC restricted. The CII reactivity of JT1 was suppressed by phermacological dose of estradiol. We therefore investigated the estrogen receptor (ER) expressing cells in rheumatoid synovium, and found that hyperplastic synovial lining cells are expressing ER.We subsequently examined the ER expression of the articular cartilage, and found the ER mRNA in chondrocytes. Numerous attempts to establish hybridomas of JT1 and Jurkat (human T cell leukemia cell line) has been unsuccessful because of unstable nature of hybridoma. We are planning to make DNA analysis of T cell receptor of JT1.

通过IL-2刺激建立II型胶原（CII）反应性T细胞克隆（JT 1）。这种对CII的反应性被证明是MHC限制性的。JT 1的CII反应性可被雌二醇抑制。因此，我们研究了雌激素受体（ER）表达细胞在类风湿性关节炎滑膜，并发现增生的滑膜衬里细胞表达ER。我们随后检查了ER的表达的关节软骨，并发现ER mRNA的软骨细胞。建立JT 1和Jurkat（人T细胞白血病细胞系）的杂交瘤的许多尝试由于杂交瘤的不稳定性质而不成功。我们计划对JT 1的T细胞受体进行DNA分析。

项目成果

Toshio Ushiyama,Koji Inoue,et al.: "Expression of estrogen recepto related protein (p29) and estradiol binding in human arthritic synovium." Journal of Rheumatology. 22. 421-426 (1995)

Toshio Ushiyama、Koji Inoue 等人：“雌激素受体相关蛋白 (p29) 的表达和人关节炎滑膜中雌二醇的结合。”

Toshio Ushiyama et al.: "Expression of estrogen recepto related protein (p29) and estradiol binding in human arthritic synovium." Journal of Rheumatology. Vol 22. 421-426 (1955)

Toshio Ushiyama 等人：“雌激素受体相关蛋白 (p29) 的表达和人关节炎滑膜中雌二醇的结合。”

Toshio Ushiyama,Koji Inoue,Junichi Nishioka: "Expression of estrogen receptor-related protein (P29)and estradid binding in human arthritic synovium." The Journal of Rheumatology. 22(in press). (1995)

Toshio Ushiyama、Koji Inoue、Junichi Nishioka：“雌激素受体相关蛋白 (P29) 的表达和人关节炎滑膜中雌激素的结合。”

Toshio Ushiyama et al.: "Expression of estrogen recepto related protein(P29)and estradiol binding in human arthritic synovium" Journal of Rheumatology. 22. 421-426 (1995)

Toshio Ushiyama 等人：“人关节炎滑膜中雌激素受体相关蛋白 (P29) 的表达和雌二醇结合”风湿病学杂志。