Clinical studies of adoptive immunochemotherapy combined with anti-cancer agents in the treatment of malignant bone tumor

为了评价动脉和静脉注射抗癌药物后的生物学反应，我们研究了血清细胞因子水平的变化。采用ELISA法检测4种细胞因子（il -1 β、IL-2、IL-6、tnf - α）。静脉输注抗癌药物后，观察到IL-6和tnf - α的产生显著增加。动脉内输注药物后，这些细胞因子的产生略有增加。另一方面，注射这些药物后，il -1 β和IL-2水平没有变化。输注后白细胞介素-6和tnf - α几乎同时升高，白细胞数量下降。这种现象在表现良好的病人身上表现得很明显。过继性免疫化疗联合抗癌药物治疗恶性骨肿瘤的疗效有待进一步研究。

In order to evaluate the biological response after intraarterial and intravenous administration anti-cancer agents, we studied the changes of cytokine levels in serum.Four types of cytokines (IL-1beta, IL-2, IL-6, TNF-alpha) were measured by ELISA method. A significant increase of IL-6 and TNF-alpha production was observed after intravenous infusion of anti-cancer agents.After intraarterial infusion of agents there was a slight increase in the production of these cytokines.On the other hand, there were no changes in the IL-1beta and IL-2 levels after infusion of the agents.IL-6 and TNF-alpha increased almost at the same time that the numbers of WBC decreased after infusion.And this phenomenon was clear in the patients who had a good performance status.Further studies should be done to confirm the efficacy of the adoptive immunochemotherapy combined with anti-cancer agents for malignat bone tumor.