Genomics of eco-evolutionary dynamics in coevolving predator-prey systems

共同进化的捕食者-被捕食者系统中生态进化动力学的基因组学

• 批准号: 432982237
• 负责人: Professor Dr. Lutz Becks
• 金额: --
• 依托单位: Limnologisches Institut
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/432982237?language=en
• 关键词: Genomics; eco; evolutionary; dynamics; coevolving

Reciprocal evolutionary changes of interacting species are major drivers for diversification and speciation and have mainly been studied in host-parasite systems and for mutualistic interactions. Although predator-prey interactions are commonplace and characterized by strong selection from both sides, predator-prey coevolution and its consequences on diversification have rarely been studied and never on the sequence level. Here we propose to combine a long-term experimental evolution study with whole genome sequencing of microbial populations over time. We allowed 7 bacterial species to evolve in replicate cultures in the absence and in the presence of the ciliate Tetrahymena thermophila (predator) for 34 months. We have already collected data on the population sizes over time and started to assay evolutionary changes in the prey and predator on the phenotype level of prey (growth and defense against ciliate) and predator (growth and offence) from preserved samples. Within the proposed project, we plan to sequence predator and prey populations from multiple time points. Based on previous work, we aim to identify adaptive allele changes, follow allele trajectories over time, compare within and between species changes in the prey, correlate changes in the prey and predator population and finally link these dynamics to the phenotypic and population size changes. These will be the first data following predator-prey coevolution on the genome level including information on the whole genome and for whole populations over time. The experimental design allows us further to compare evolutionary changes within replicates of the same species as well as across species, giving us the unique opportunity to assess the repeatability of evolutionary change in terms of mutations arsing and their trajectory as well as in terms of patterns of evolutionary change, such as the occurrence of selective sweeps, clonal interference or frequency dependent selection.

相互作用物种的相互进化变化是多样化和物种形成的主要驱动力，主要在宿主-寄生虫系统和互利相互作用中进行了研究。虽然捕食者-猎物的相互作用是司空见惯的，其特点是从双方的强选择，捕食者-猎物的共同进化及其后果的多样化很少被研究，从来没有在序列水平上。在这里，我们建议结合联合收割机的长期实验进化研究与全基因组测序的微生物种群随着时间的推移。我们允许7个细菌物种在重复培养物中进化，在存在和不存在纤毛四膜虫（捕食者）的情况下，持续34个月。我们已经收集了随着时间的推移种群规模的数据，并开始分析猎物和捕食者的进化变化的表型水平的猎物（生长和防御纤毛虫）和捕食者（生长和进攻）从保存的样品。在拟议的项目中，我们计划从多个时间点对捕食者和猎物种群进行排序。基于以前的工作，我们的目标是确定适应性等位基因的变化，随着时间的推移，遵循等位基因轨迹，比较内和物种之间的猎物的变化，相关的猎物和捕食者人口的变化，并最终将这些动态的表型和人口规模的变化。这些将是在基因组水平上捕食者-猎物共同进化之后的第一批数据，包括整个基因组和整个种群随时间推移的信息。实验设计使我们能够进一步比较同一物种的重复内以及跨物种的进化变化，使我们有独特的机会来评估突变arsing和它们的轨迹方面的进化变化的可重复性，以及在进化变化的模式，如发生选择性扫描，克隆干扰或频率依赖性选择。