ANALGESIC EFFECTS IN DOGS AFTER INTRTHECAL ND EPIDURAL INJECTION OF ADENOSINE TRIPHOSPHATE AND ADENOSINE ANALOG.

鞘内和硬膜外注射三磷酸腺苷和腺苷类似物后对狗的镇痛作用。

• 批准号: 05671271
• 负责人: HARANO Kiyoshi
• 金额: $ 1.41万
• 依托单位: SAGA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671271/
• 关键词: ADENOSINE TRIPHOSPHATE; ADENOSINE ANALOG; R-PHENYLISOPROPYL-ADENOSINE; INTRATHECAL INJECTION; EPIDURAL NJECTION; PAIN; アデノシン; 脊椎麻酔; 硬膜外麻酔; アデノシンアナログ; 脊髄麻酔

Within a few minutes after lumbar intrathecal injection of adenosine triphosphate (ATP,40mg/2ml), the cardiovascular responses were suppressed in the dogs under light anesthesia with isoflurane in oxygene. The intrathecal injection of methylxantine (aminophilline, 50mg/2ml) make the dogs alart as noxicious as the control. The same effect will longer duration was obtained if we use 1muM/2ml of R-phenylisopropyl-adenosine, one of the adenosine analogs which acts selectively to A1 adenosine receptor. In this case, antinociceptive effect was last for 40-90 minutesIn the unanesthetized dog, the motor dysfunction was appeared in the hindlimb and last for 3 hours after intrathecal ATP injection.In the case of epidural injection of the same drugs, the antinociceptive effect was smaller than that of intrathecal injection, even the 1.5times higher dosage were applied.Adenosine is an endogeneous nucleoside occuring in all cell of the body. Adenosine is also involved in nociception and transmission through the spinal cord.If the analgesia coud be obtained with adenosine or its analogs, it should be one of the ideal analgesics.We demonstrated the analgesic effect of the ATP and R-PIA for the pain stimuli in dogs.

犬腰段鞘内注射三磷酸腺苷（ATP，40 mg/2 ml）后，在几分钟内，心血管反应被抑制。鞘内注射甲基黄嘌呤（氨酚林，50 mg/2 ml）使犬产生与对照组相同的毒性反应。如果我们使用1 μ M/2 ml的R-苯异丙基-腺苷，则可以获得相同的效果，并且持续时间更长，R-苯异丙基-腺苷是选择性作用于A1腺苷受体的腺苷类似物之一。在未麻醉的狗中，鞘内注射ATP后，后肢出现运动功能障碍，并持续3小时，硬膜外注射相同药物的抗伤害效应小于鞘内注射，腺苷是一种内源性核苷，存在于人体的所有细胞中。腺苷也参与伤害性感受和脊髓传递，如果腺苷或其类似物能产生镇痛作用，它将是一种理想的镇痛剂。

项目成果

高崎光浩, 原野 清, 谷口良雄, 十時忠秀: "DPPCベシクル中の疎水性環境に対するアデノシン3リン酸添加の効果21GC01:麻酔" 42. S740- (1993)

Mitsuhiro Takasaki、Kiyoshi Harano、Yoshio Taniguchi、Tadahide Totoki：“添加三磷酸腺苷对 DPPC 囊泡 21GC01 疏水环境的影响：麻醉”42. S740- (1993)