Immunodetection and immunotherapy for metastatic testicular germ cell tumors using antiplacental alkaline phosphatase monoclonal antibody

使用抗胎盘碱性磷酸酶单克隆抗体对转移性睾丸生殖细胞肿瘤进行免疫检测和免疫治疗

• 批准号: 05671307
• 负责人: KOSHIDA Kiyoshi
• 金额: $ 1.47万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671307/
• 关键词: Testicular tumor; Placental alkaline phosphatase; Radioimmunotherapy; モノクローナル抗体; 腫瘍イメージング

We investigated significance of placental alkaline phosphase (PLAP) as a tumor marker for seminoma, and assessed a potential of PLAP as a targeting antigen for immunodetection and immunotherapy using anti-PLAP MAb. The monitoring of serum PLAP might be of value, as fluctuations in this marker provide information adout disease status and prognosis. Specific localization of anti-PLAP MAb to PLAP-producing xenografts was confirmed in a nude mouse model. In a SCID mouse model of testicular tumor and its lymph node metastases, localization of the MAb in lymph node metastases was more efficient than localization in testis tumors, suggesting the potential use of anti-PLAP MAb for radioimmunotherapy (RIT) on small metastatic foci of seminoma. However, efficacy of RIT on the metastaitc foci remains to be clarified since complete elimination of tumorcells was unlikely to occur in the mouse model treated with I-131 labeled anti-PLAP MAb.

我们研究了胎盘碱性磷酸酶（PLAP）作为乳腺癌肿瘤标志物的意义，并评估了PLAP作为免疫检测和免疫治疗的靶向抗原的潜力。监测血清PLAP可能有价值，因为该标志物的波动提供了疾病状态和预后的信息。在裸鼠模型中证实了抗PLAP MAb对产生PLAP的异种移植物的特异性定位。在睾丸肿瘤及其淋巴结转移的SCID小鼠模型中，MAb在淋巴结转移中的定位比在睾丸肿瘤中的定位更有效，这表明抗PLAP MAb用于放射免疫治疗（RIT）的潜在用途。然而，RIT对转移灶的疗效仍有待澄清，因为在用I-131标记的抗PLAP单抗处理的小鼠模型中不太可能发生肿瘤细胞的完全消除。

项目成果

K.Koshida et al: "A potential use et a wouodoual antihody xo phceutal alkeline plasplutwe to detect lymyh hide metaltases of somiusma" Journal of Uvology. 155. 337-341 (1996)

K.Koshida 等人：“一种潜在的用途 et a wouodoual antihody xo phceutal alkeline plasplutwe 来检测 somiusma 的 lymyh 皮金属酶”Uvology 杂志。

K.Koshida et al.: "A potential ceie of a inonoclonal autibody to placoutal allcaline phosphatnse(PLAP)to detect lymph uoclo metrstusos of seminowa." J.Urology. (accepted).

K.Koshida 等人：“一种潜在的单克隆抗体对 placoutal 碱性磷酸酶 (PLAP) 的检测，可用于检测 Seminowa 的淋巴转移。”

K.Koshida et al.: "A potmtiol uca y a monoclonal ontihody to plocental allcoline plsnhotme todect lyngh hode metaltular of Sewinoma" Journal of Urology. 155. 337-341 (1996)

K.Koshida 等人：“A potmtiol uca y a monoclonal ontihody to plocental allcoline plsnhotme todect lyngh hode metaltular of Sewinoma”泌尿学杂志。

Yamamoto,H.et al.: "Immunopathology of Allcaline Phosphatuse Isozymes in Seminoma" Urologia Internationalis. 50. 33-35 (1993)

Yamamoto,H.et al.：“精原细胞瘤中碱性磷酸酶同工酶的免疫病理学”Urologia Internationalis。

K.Koshida, T.Uchibayashi, H.Yamamoto, K.Hirano.: "Significance of placental alkaline phosphatase (PLAP) in the monitoring of patients with seminoma." British Journal of Urology. 77. 138-142 (1996)

K.Koshida、T.Uchibayashi、H.Yamamoto、K.Hirano.：“胎盘碱性磷酸酶 (PLAP) 在监测精原细胞瘤患者中的意义。”