Immunodetection and immunotherapy for metastatic testicular germ cell tumors using antiplacental alkaline phosphatase monoclonal antibody

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We investigated significance of placental alkaline phosphase (PLAP) as a tumor marker for seminoma, and assessed a potential of PLAP as a targeting antigen for immunodetection and immunotherapy using anti-PLAP MAb. The monitoring of serum PLAP might be of value, as fluctuations in this marker provide information adout disease status and prognosis. Specific localization of anti-PLAP MAb to PLAP-producing xenografts was confirmed in a nude mouse model. In a SCID mouse model of testicular tumor and its lymph node metastases, localization of the MAb in lymph node metastases was more efficient than localization in testis tumors, suggesting the potential use of anti-PLAP MAb for radioimmunotherapy (RIT) on small metastatic foci of seminoma. However, efficacy of RIT on the metastaitc foci remains to be clarified since complete elimination of tumorcells was unlikely to occur in the mouse model treated with I-131 labeled anti-PLAP MAb.