Relationship between gastric carcinoma and intestinal metaplasia from the aspect of the expression of placental alkaline phosphatase messenger RNA

从胎盘碱性磷酸酶信使RNA表达角度探讨胃癌与肠化生的关系

• 批准号: 02670301
• 负责人: OKAI Takashi
• 金额: $ 1.28万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670301/
• 关键词: Placental alkaline phosphatase (PLAP); immunohistochemistry; PLAP mRNA; RT-PCR method; gastric cancer; KATO-III; intestinal mucosal tissue; PCR; hybridization; mRNA

Intestinal metaplasia of the gastric mucosa has been suggested to represent a precancerous state. However, how it is related to carcinogenesis remains controversial. We developed specific monoclonal antibodies (MAbs) against placental alkaline phosphatase (PLAP). Imnninohistochemical study with the specific MAb showed that expression of PLAP was apt to occur in more highly differentiated gastric carcinoma, and was highly specific for carcinoma. Therefore, we attempted to elucidate the expression of PLAP MRNA with the RT-PCR method in various benign and malignant gastric diseases. Each RNA was extracted from KATO-III cells (gastric cancer), intestinal mucosal tissue, BeWo cells (chorionic carcinoma), and placental tissue. Primer pairs were selected as synthetic 25 oligonucleotides which detected the PLAP cDNA region (2l4 bp) which was widely different from intestinal alkaline phosphatase cDNA (IAP cDNA). The results of RT-PCR showed that 214 bp band appeared in samples from KATO-III cells, BeWo cells, intestinal mucosal tissue as well as placental tissue. On the other hand, PLAP cDNA is very similar to IAP cDNA. These results indicated that primer pairs probably reacted with both PLAP mRNA and IAP mRNA. Futhermore, the amplified PLAPcDNA region in the sample of KATO- III was recognized as a slightly small weight band in electrophoresis and therefore several base pairs of the amplified region might be deleted in KATO- III cells. To clarify these points, further investigations continue.

胃粘膜的肠上皮化生被认为是癌前状态。然而，它与致癌作用的关系仍然存在争议。我们开发了针对胎盘碱性磷酸酶（PLAP）的特异性单克隆抗体（MAbs）。免疫组化结果显示PLAP在分化程度较高的胃癌中表达，且具有高度的特异性。因此，我们试图用RT-PCR方法来阐明PLAP mRNA在各种胃良恶性疾病中的表达。从KATO-III细胞（胃癌）、肠粘膜组织、BeWo细胞（绒毛膜癌）和胎盘组织中提取各RNA。选择引物对作为合成的25个寡核苷酸，其检测PLAP cDNA区域（214 bp），该区域与肠碱性磷酸酶cDNA（IAP cDNA）广泛不同。RT-PCR结果显示，在KATO-Ⅲ细胞、BeWo细胞、肠粘膜组织和胎盘组织中均出现214 bp的条带。另一方面，PLAP cDNA与IAP cDNA非常相似。这些结果表明，引物对可能与PLAP mRNA和IAP mRNA反应。因此，在KATO-Ⅲ细胞中扩增的PLAPcDNA区在电泳中被识别为一个稍小的重量带，因此在KATO-Ⅲ细胞中扩增区的几个碱基对可能被删除。为了澄清这些问题，进一步的调查仍在继续。

项目成果

H. Watanabe, Y. Yamaguchi, I. Mouri, O. Yamakawa, H. Kawakami, Y. Satomura, H. Ohta, Y. Motoo, T. Okai, N. Sawabu: "Significance of detecting the tumor-associated antigens[placental alkaline phosphatase (PLAP), ST-439, and Sialyl SSEA-1 (SLX)] on the diff

H. Watanabe、Y. Yamaguchi、I. Mouri、O. Yamakawa、H. Kawakami、Y. Satomura、H. Ohta、Y. Motoo、T. Okai、N. Sawabu：“检测肿瘤相关抗原的意义[

Watanabe H: "Singificance of detecting the tumor-associated antigens 〔placental alkaline phosphatase(PLAP),ST-439,and Sialyl SSEA-1(SLX)〕on the differential diagnosis of bening or malignant disease with the metarials of gastric biopsies" Cancer.

Watanabe H：“检测肿瘤相关抗原 [胎盘碱性磷酸酶 (PLAP)、ST-439 和唾液酸 SSEA-1 (SLX)] 对胃活检金属元素鉴别诊断良性或恶性疾病的意义” 癌症。

渡辺 弘之: "胎盤型アルカリフォスファタ-ゼ[PLAP]の発現より胃腸上皮化生と胃癌との関連" Prog.Med. 10. 2373-2380 (1990)

Hiroyuki Watanabe：“基于胎盘碱性磷酸酶 [PLAP] 表达的胃肠道化生与胃癌之间的关系”Prog.Med. 10. 2373-2380 (1990)

渡辺 弘之: "胎盤型アルカリフォスファタ-ゼ〔PLAP〕の発現よりみた胃腸上皮化主と胃癌の関連" Prog.Med. 10. 2373-2380 (1990)

Hiroyuki Watanabe：“基于胎盘碱性磷酸酶 [PLAP] 表达的胃肠道上皮化与胃癌之间的关系”Prog.Med. 10. 2373-2380 (1990)

T. Okai, O. Yamakawa, N. Matsuda, H. Kawakami, H. Watanabe, Y. Satomura, H. Ohta, Y. Motoo, N, Sawabu: "Analysis of gastric carcinoma growth by endoscopic ultrasonography." Endoscopy. 23. 121-125 (1991)

T. Okai、O. Yamakawa、N. Matsuda、H. Kawakami、H. Watanabe、Y. Satomura、H. Ohta、Y. Motoo、N、Sawabu：“通过内窥镜超声分析胃癌生长。”